製品の概要

  • 製品名Anti-ErbB 4 antibody [HFR-1]
    ErbB 4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HFR-1] to ErbB 4
  • アプリケーション適用あり: Flow Cyt, ICC/IF, WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Chicken
  • 免疫原

    Synthetic peptide:

    (C)RSTLQHPDYLQEYST

    , corresponding to amino acids 1250-1264 of HER4.

  • ポジティブ・コントロール
    • Human A431 cell and mouse NIH3T3/HER4 cell extracts (western blot). NIH3T3 cells (IHC-P).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab19391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 2µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB Use a concentration of 3 µg/ml. Detects a band of approximately 185 kDa (predicted molecular weight: 147 kDa).
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 6 - 8 µg/ml.

ターゲット情報

  • 機能Specifically binds and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. The C-terminal fragment (CTF) of isoform JMA-A CYT-2 (containing E4ICD2) can stimulate transcription in the presence of YAP1. ERBB4 intracellular domain is involved in the regulation of cell growth. Conflicting reports are likely due at least in part to the opposing effects of the isoform-specific and nuclear-translocated ERBB4 intracellular domains (E4ICD1 and E4ICD2). Overexpression studies in epithelium show growth inhibition using E4ICD1 and increased proliferation using E4ICD2. E4ICD2 has greater in vitro kinase activity than E4ICD1. The kinase activity is required for the nuclear translocation of E4ICD2.
  • 組織特異性Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are expressed in cerebellum, but only the isoform JM-B is expressed in the heart.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Isoform JM-A CYT-1 and isoform JM-A CYT-2 but not isoform JM-B CYT-1 and isoform JM-B CYT-2 are processed by ADAM17. Proteolytic processing in response to ligand or 12-O-tetradecanoylphorbol-13-acetate stimulation results in the production of 120 kDa soluble receptor forms and intermediate membrane-anchored 80 kDa fragments (m80HER4), which are further processed by a presenilin-dependent gamma-secretase to release the respective cytoplasmic intracellular domain E4ICD (either E4ICD1/s80Cyt1 or E4ICD2/s80Cyt2). Membrane-anchored 80 kDa fragments of the processed isoform JM-A CYT-1 are more readily degraded by the proteasome than fragments of isoform JM-A CYT-2 suggesting a prevalence of E4ICD2 over E4ICD1.
    Ligand-binding increases phosphorylation on tyrosine residues. Isoform JM-A CYT-2 is constitutively phosphorylated on tyrosine residues in a ligand-independent manner. E4ICD2 but not E4ICD1 is phosphorylated on tyrosine residues.
    Ubiquitinated. The ERBB4 intracellular domain is ubiquitinated and targeted to proteosomal degradation during mitosis mediated by the APC/C complex. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are ubiquitinated by WWP1. The ERBB4 intracellular domain (E4ICD1) is ubiquitinated, and this involves NEDD4.
  • 細胞内局在Membrane and Nucleus. Following proteolytical processing E4ICD (E4ICD1 or E4ICD2 generated from the respective isoforms) is translocated to the nucleus. Significantly more E4ICD2 than E4ICD1 is found in the nucleus. E4ICD2 colocalizes with YAP1 in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4ICD antibody
    • ALS19 antibody
    • Avian erythroblastic leukemia viral oncogene homolog 4 antibody
    • Avian erythroblastic leukemia viral v erb b2 oncogene homolog 4 antibody
    • E4ICD antibody
    • EC 2.7.10.1 antibody
    • Erbb4 antibody
    • ERBB4 intracellular domain antibody
    • ERBB4_HUMAN antibody
    • HER 4 antibody
    • HER4 antibody
    • human epidermal growth factor receptor 4 antibody
    • Mer4 antibody
    • MGC138404 antibody
    • Oncogene ERBB4 antibody
    • p180erbB4 antibody
    • Proto-oncogene-like protein c-ErbB-4 antibody
    • Receptor protein tyrosine kinase erbB 4 precursor antibody
    • Receptor tyrosine protein kinase erbB 4 antibody
    • s80HER4 antibody
    • Tyrosine kinase type cell surface receptor HER4 antibody
    • Tyrosine kinase-type cell surface receptor HER4 antibody
    • v erb a avian erythroblastic leukemia viral oncogene homolog like 4 antibody
    • v erb a erythroblastic leukemia viral oncogene homolog 4 antibody
    • v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) antibody
    • V-ERB-B2 avian erythroblastic leukemia viral oncogene homolog 4 antibody
    • Verba avian erythroblastic leukemia viral oncogene homolog like 4 antibody
    • Verba erythroblastic leukemia viral oncogene homolog 4 antibody
    • VERBB2 antibody
    see all

Anti-ErbB 4 antibody [HFR-1] 画像

  • ICC/IF image of ab19391 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19391, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HEK293 cells stained with ab19391 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19391, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human Breast carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing ErbB4 (ab19391) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human Heart tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing ErbB4 (ab19391) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human Pancreas tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing ErbB4 (ab193911) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • ab19391 staining ErbB 4 in Mouse brain tissue by Immunohistochemistry (Frozen sections). The sections were fixed with paraformaldehyde. The primary antibody was used neat and incubated with the sample for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-Mouse polyclonal was used as the secondary antibody.

    See Abreview

Anti-ErbB 4 antibody [HFR-1] (ab19391) 使用論文

This product has been referenced in:
  • Gruessner C  et al. Biomarkers and endosalpingiosis in the ovarian and tubal microenvironment of women at high-risk for pelvic serous carcinoma. Am J Cancer Res 4:61-72 (2014). IHC-P ; Human . Read more (PubMed: 24482739) »
  • Gruessner C  et al. Flutamide and biomarkers in women at high risk for ovarian cancer: preclinical and clinical evidence. Cancer Prev Res (Phila) 7:896-905 (2014). Read more (PubMed: 24950779) »

See all 3 Publications for this product

Product Wall

Thank you for your telephone call earlier today. According to Swissprot/Uniprot protein database site, the extracellular domain of the ErbB 4 is from amino acids 26 – 651. http://www.uniprot.org/uniprot/Q15303 We do have the following ...

Read More
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Permeabilization No
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Abcam user community

Verified customer

投稿 Mar 28 2011

Thank you for your enquiry. I have checked with the originator of the product but unfortunately, they do not have an image of the IHC stain done with this antibody. However, they have provided some references using a similar clone and I have attache...

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