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RabMAb

Anti-ErbB 4 抗体 [E200] (ab32375)

製品の概要

  • 製品名
    Anti-ErbB 4 antibody [E200]
    ErbB 4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E200] to ErbB 4
  • 特異性
    This antibody is specific to ErbB 4. It does not cross react with other EGF receptor family members.
  • アプリケーション
    適用あり: WB, ICC, Flow Cyt, IPmore details
    適用なし: IHC
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human ErbB 4 (C terminal).

  • ポジティブ・コントロール
    • MCF7 cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Alternative versions available:

    Anti-ErbB 4 antibody (Alexa Fluor® 647) [E200] (ab200518)

    Anti-ErbB 4 antibody (Alexa Fluor® 488) [E200] (ab200517)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 147 kDa.
ICC 1/50.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/100.
  • 追加情報
    Is unsuitable for IHC.
  • ターゲット情報

    • 機能
      Specifically binds and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. The C-terminal fragment (CTF) of isoform JMA-A CYT-2 (containing E4ICD2) can stimulate transcription in the presence of YAP1. ERBB4 intracellular domain is involved in the regulation of cell growth. Conflicting reports are likely due at least in part to the opposing effects of the isoform-specific and nuclear-translocated ERBB4 intracellular domains (E4ICD1 and E4ICD2). Overexpression studies in epithelium show growth inhibition using E4ICD1 and increased proliferation using E4ICD2. E4ICD2 has greater in vitro kinase activity than E4ICD1. The kinase activity is required for the nuclear translocation of E4ICD2.
    • 組織特異性
      Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are expressed in cerebellum, but only the isoform JM-B is expressed in the heart.
    • 配列類似性
      Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • 翻訳後修飾
      Isoform JM-A CYT-1 and isoform JM-A CYT-2 but not isoform JM-B CYT-1 and isoform JM-B CYT-2 are processed by ADAM17. Proteolytic processing in response to ligand or 12-O-tetradecanoylphorbol-13-acetate stimulation results in the production of 120 kDa soluble receptor forms and intermediate membrane-anchored 80 kDa fragments (m80HER4), which are further processed by a presenilin-dependent gamma-secretase to release the respective cytoplasmic intracellular domain E4ICD (either E4ICD1/s80Cyt1 or E4ICD2/s80Cyt2). Membrane-anchored 80 kDa fragments of the processed isoform JM-A CYT-1 are more readily degraded by the proteasome than fragments of isoform JM-A CYT-2 suggesting a prevalence of E4ICD2 over E4ICD1.
      Ligand-binding increases phosphorylation on tyrosine residues. Isoform JM-A CYT-2 is constitutively phosphorylated on tyrosine residues in a ligand-independent manner. E4ICD2 but not E4ICD1 is phosphorylated on tyrosine residues.
      Ubiquitinated. The ERBB4 intracellular domain is ubiquitinated and targeted to proteosomal degradation during mitosis mediated by the APC/C complex. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are ubiquitinated by WWP1. The ERBB4 intracellular domain (E4ICD1) is ubiquitinated, and this involves NEDD4.
    • 細胞内局在
      Membrane and Nucleus. Following proteolytical processing E4ICD (E4ICD1 or E4ICD2 generated from the respective isoforms) is translocated to the nucleus. Significantly more E4ICD2 than E4ICD1 is found in the nucleus. E4ICD2 colocalizes with YAP1 in the nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 4ICD antibody
      • ALS19 antibody
      • Avian erythroblastic leukemia viral oncogene homolog 4 antibody
      • Avian erythroblastic leukemia viral v erb b2 oncogene homolog 4 antibody
      • E4ICD antibody
      • EC 2.7.10.1 antibody
      • Erbb4 antibody
      • ERBB4 intracellular domain antibody
      • ERBB4_HUMAN antibody
      • HER 4 antibody
      • HER4 antibody
      • human epidermal growth factor receptor 4 antibody
      • Mer4 antibody
      • MGC138404 antibody
      • Oncogene ERBB4 antibody
      • p180erbB4 antibody
      • Proto-oncogene-like protein c-ErbB-4 antibody
      • Receptor protein tyrosine kinase erbB 4 precursor antibody
      • Receptor tyrosine protein kinase erbB 4 antibody
      • s80HER4 antibody
      • Tyrosine kinase type cell surface receptor HER4 antibody
      • Tyrosine kinase-type cell surface receptor HER4 antibody
      • v erb a avian erythroblastic leukemia viral oncogene homolog like 4 antibody
      • v erb a erythroblastic leukemia viral oncogene homolog 4 antibody
      • v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) antibody
      • V-ERB-B2 avian erythroblastic leukemia viral oncogene homolog 4 antibody
      • Verba avian erythroblastic leukemia viral oncogene homolog like 4 antibody
      • Verba erythroblastic leukemia viral oncogene homolog 4 antibody
      • VERBB2 antibody
      see all

    画像

    • Anti-ErbB 4 antibody [E200] (ab32375) at 1/4000 dilution + MCF7 cell lysate

      Predicted band size : 147 kDa
      Observed band size : 185 kDa (why is the actual band size different from the predicted?)
    • Lane 1 (input): HEK-293 (human embryonic kidney epithelial cell) whole cell lysate, 10μg
      Lane 2 (+): HEK-293 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32375 in HEK-293 whole cell lysate

      Ab32375 Immunoprecipitating ErbB 4 in HEK-293 whole cell lysate. Capture antibody was used at a 1:50 dilution (2μg in 0.35mg lysates). For western blotting, primary antibody used as ab32375 at 1:500 dilution. Ab131366 VeriBlot for IP (HRP) was used as the secondary antibody at 1:1000 dilution. The lower band at around 75kDa should be proteolysis fragment based on the literature. (PMID: 9362517)

      Blocking and diluting buffer: 5% NFDM/TBST

    参考文献

    This product has been referenced in:
    • Schumacher MA  et al. ErbB4 signaling stimulates pro-inflammatory macrophage apoptosis and limits colonic inflammation. Cell Death Dis 8:e2622 (2017). Read more (PubMed: 28230865) »
    • Jin J  et al. Mutational Analysis of Glycogen Synthase Kinase 3ß Protein Kinase Together with Kinome-Wide Binding and Stability Studies Suggests Context-Dependent Recognition of Kinases by the Chaperone Heat Shock Protein 90. Mol Cell Biol 36:1007-18 (2016). Read more (PubMed: 26755559) »

    See all 11 Publications for this product

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Breast Cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
    Permeabilization
    Yes - Wash Buffer from Dako with tween
    Specification
    Breast Cancer
    Fixative
    Paraformaldehyde
    Username

    Mr. Rudolf Jung

    Verified customer

    投稿 Dec 10 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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