製品の概要
- 製品名Anti-ErbB 2 antibody, prediluted
ErbB 2 一次抗体 製品一覧 - 製品の詳細Rabbit polyclonal to ErbB 2, prediluted
- 特異性This antibody is specific against human c-erbB-2 oncoprotein.
- アプリケーション適用あり: IHC-FoFr, IHC-P, IHC-Frmore details
- 種交差性交差種: Human
交差が予測される動物種: Mouse, Rat
- 免疫原
Synthetic human c-erbB-2 oncoprotein from intracytoplasmatic part of the c-erbB-2 oncoprotein.
- ポジティブ･コントロール
- Breast Adenocarcinomas, ovary, gastrointestinal tract.
- 特記事項
This antibody labels epithelial cells in approximately 20% of cases of adenocarcinomas from various sites such as breast, ovary, and gastrointestinal tract.
製品の特性
- 製品の状態Liquid
- 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- バッファー0.15m NaCl, 0.05% sodium azide, 0.001% green food dye, ph7.6.
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Concentration information loading... - 精製度IgG fraction
- 一次抗体 備考This antibody labels epithelial cells in approximately 20% of cases of adenocarcinomas from various sites such as breast, ovary, and gastrointestinal tract.
- ポリ/モノポリクローナル
- アイソタイプIgG
- 研究分野
関連製品
- Affibody® Molecule
- Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab8801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-FoFr | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Prolonged fixation in buffered formalin can destroy the epitope. We recommend an incubation time and temperature of 30 mintues at 37°C when used in conjunction with immunoperoxidase staining systems. For consistent and reproducible results, we recommend using a high temperature antigenic unmasking technique for formalin-fixed, paraffin-embedded tissues. |
|
| IHC-Fr | Use at an assay dependent concentration. |
ターゲット情報
- 機能Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. - 組織特異性Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
- 関連疾患Hereditary diffuse gastric cancer
Glioma
Ovarian cancer
Lung cancer
Gastric cancer
Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2. - 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. - 翻訳後修飾Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172).
- 細胞内局在Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
- Information by UniProt
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参照データベース
- Entrez Gene: 2064 Human
- Entrez Gene: 13866 Mouse
- Entrez Gene: 24337 Rat
- Omim: 164870 Human
- SwissProt: P04626 Human
- SwissProt: P70424 Mouse
- SwissProt: P06494 Rat
- Unigene: 446352 Human
see all -
別名
- Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
- C erb B2/neu protein antibody
- CD340 antibody
see all
Anti-ErbB 2 antibody, prediluted (ab8801) 使用論文
ab8801 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"