製品の概要

  • 製品名Anti-ErbB 2 antibody [3B5]
    ErbB 2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3B5] to ErbB 2
  • アプリケーション適用あり: IHC-Fr, IHC-P, WB, IP, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Monkey
    交差が予測される動物種: Dog
  • 免疫原

    Synthetic peptide:

    TAENPEYLGLDVPV

    , corresponding to C terminal amino acids 1242 - 1255 of Human c-ErbB2.

  • エピトープWithin amino acids 1242 - 1255 of human c-neu (TAENPEYLGLDVPV)
  • ポジティブ・コントロール
    • SK-BR-3 cells or breast carcinoma tissue
  • 特記事項For paraffin sections, we recommend pretreating with a pressure cooker, but trypsin and heat will also work well. Including 0.05% saponin during staining of paraffin sections may help reduce background. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribriform growth type were stained by this antibody. MO 01/02/07: update consignment level to 0 following settlement of consignment stock in January 07.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use a concentration of 2.5 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 190 kDa (predicted molecular weight: 138 kDa).
IP Use 1µg for 106 cells.
Flow Cyt 1/50 - 1/100.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
    In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
  • 組織特異性Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
  • 関連疾患Hereditary diffuse gastric cancer
    Glioma
    Ovarian cancer
    Lung cancer
    Gastric cancer
    Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172).
  • 細胞内局在Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
    • C erb B2/neu protein antibody
    • CD340 antibody
    • CD340 antigen antibody
    • Cerb B2/neu protein antibody
    • CerbB2 antibody
    • Erb b2 receptor tyrosine kinase 2 antibody
    • ERBB2 antibody
    • ERBB2_HUMAN antibody
    • HER 2 antibody
    • HER 2/NEU antibody
    • HER2 antibody
    • Herstatin antibody
    • Human epidermal growth factor receptor 2 antibody
    • Metastatic lymph node gene 19 protein antibody
    • MLN 19 antibody
    • MLN19 antibody
    • NEU antibody
    • NEU proto oncogene antibody
    • Neuro/glioblastoma derived oncogene homolog antibody
    • Neuroblastoma/glioblastoma derived oncogene homolog antibody
    • NGL antibody
    • p185erbB2 antibody
    • Proto-oncogene c-ErbB-2 antibody
    • Proto-oncogene Neu antibody
    • Receptor tyrosine-protein kinase erbB-2 antibody
    • TKR1 antibody
    • Tyrosine kinase type cell surface receptor HER2 antibody
    • Tyrosine kinase-type cell surface receptor HER2 antibody
    • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody
    • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 antibody
    • V erb b2 avian erythroblastic leukemia viral oncoprotein 2 antibody
    • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
    • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
    • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
    see all

Anti-ErbB 2 antibody [3B5] 画像

  • Anti-ErbB 2 antibody [3B5] (ab16901) at 1 µg/ml + Whole cell lysate HEK293

    Predicted band size : 138 kDa
  • Overlay histogram showing LoVo cells stained with ab16901 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16901, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in LoVo cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

  • Immunohistochemistry on paraffin section of human breast carcinoma
  • Immunohistochemistry on paraffin section of human Mamma tumor Her2Neu +

Anti-ErbB 2 antibody [3B5] (ab16901) 使用論文

This product has been referenced in:
  • Lai Q  et al. Positive correlation between the expression of hEag1 and HIF-1a in breast cancers: an observational study. BMJ Open 4:e005049 (2014). IHC-P ; Human . Read more (PubMed: 24838727) »
  • Ward TM  et al. Truncated p110 ERBB2 induces mammary epithelial cell migration, invasion and orthotopic xenograft formation, and is associated with loss of phosphorylated STAT5. Oncogene : (2012). WB ; Human . Read more (PubMed: 22751112) »

See all 6 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (N87 cell line)
Permeabilization No
Gating Strategy no gating
Specification N87 cell line
Preparation Cell harvesting/tissue preparation method: Cells scrapped in PBS
Sample buffer: PBS with 0.5% BSA
Fixation Formaldehyde
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投稿 Jul 06 2016

Application Western blot
Sample Human Cell lysate - whole cell (N87 cells)
Gel Running Conditions Reduced Denaturing (10% Tris Glycin gel)
Loading amount 400000 cells
Specification N87 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Jun 22 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human breast cancer)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Acetate pH6
Permeabilization No
Specification human breast cancer
Blocking step Innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative Formaldehyde
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投稿 Jun 15 2016

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Huh7 (liver))
Specification Huh7 (liver)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Apr 30 2014



It does sound like the buffer additives are interfering with the conjugation reaction. The gelatin and azide contain free amine groups that will interfere with this kind of reaction. I would suggest doing a buffer exchange or using a purifica...

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Thank you for your enquiry.

I can confirm that ErbB2 is known to be expressed in breast issue and highly expressed in many breast cancers. Breast tumor tissue would therefore be a positive control to consider. You may want to do consider som...

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Thank you for contacting us. I have included information about the ErbB1 peptides and antibodies below. In addition I have attached our ELISA protocols and further information about direct labeling of our antibodies as requested.

http://www....

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Thank you for contacting us. The peptide catalog ab38955 does contain the TAENPEYLGLDVPV (aa 1242-1255) sequence, plus a few additional amino acids.

I hope this information is helpful to you. Please do not hesitate to contact us if you need an...

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Thank you for getting back to me in regards to this complaint.


As discussed previously, the band at ˜170 kDa may well be the specific band for ErbB 2 as the protein goes through post translational modification to increase its weight. In...

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