製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to ERAP2
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Orangutan
  • 免疫原

    Peptide with sequence C-

    QYLKKFSYRNAKND

    , corresponding to internal sequence amino acids 480-493 of human ERAP2 (NP_071745.1).

  • ポジティブ・コントロール
    • Human spleen lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:16,000.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 110 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      ERAP2 is an aminopeptidase that plays a central role in peptide trimming. Major histocompatibility complex (MHC) class I molecules rely on aminopeptidases such as ERAP2 to trim precursors to antigenic peptides in the endoplasmic reticulum (ER) following cleavage in the cytoplasm by tripeptidyl peptidase II. ERAP2 preferentially hydrolyzes the basic residues Arg and Lys.
    • 細胞内局在
      Endoplasmic reticulum membrane; Single-pass type II membrane protein.
    • 参照データベース
    • 別名
      • Endoplasmic reticulum aminopeptidase 2 antibody
      • L RAP antibody
      • Leukocyte derived arginine aminopeptidase antibody
      • LRAP antibody

    画像

    • Anti-ERAP2 antibody (ab82142) at 0.5 µg/ml + human spleen lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 110 kDa
      Observed band size : 110 kDa
      Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 1 hour

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    参考文献

    ab82142 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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