製品の概要

  • 製品名Anti-ERAP2 antibody
    ERAP2 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to ERAP2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human ERAP2 protein (AAH17927, 350 a.a)

  • ポジティブ・コントロール
    • Human kidney tissue lysate. ERAP2 transfected 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/500 - 1/1000. Detects bands of approximately 110 and 40 kDa (predicted molecular weight: 110 kDa; Isoform 4, 40 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性ERAP2 is an aminopeptidase that plays a central role in peptide trimming. Major histocompatibility complex (MHC) class I molecules rely on aminopeptidases such as ERAP2 to trim precursors to antigenic peptides in the endoplasmic reticulum (ER) following cleavage in the cytoplasm by tripeptidyl peptidase II. ERAP2 preferentially hydrolyzes the basic residues Arg and Lys.
    • 細胞内局在Endoplasmic reticulum membrane; Single-pass type II membrane protein.
    • 参照データベース
    • 別名
      • Endoplasmic reticulum aminopeptidase 2 antibody
      • L RAP antibody
      • Leukocyte derived arginine aminopeptidase antibody
      • LRAP antibody

    Anti-ERAP2 antibody 画像

    • Anti-ERAP2 antibody (ab69037) at 1/500 dilution + Human kidney lysate at 50 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 110 kDa
      Observed band size : 110 kDa
    • All lanes : Anti-ERAP2 antibody (ab69037) at 1/500 dilution

      Lane 1 : ERAP2 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 110 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)

    Anti-ERAP2 antibody (ab69037) 使用論文

    This product has been referenced in:
    • Kuiper JJ  et al. A genome-wide association study identifies a functional ERAP2 haplotype associated with birdshot chorioretinopathy. Hum Mol Genet 23:6081-7 (2014). Human . Read more (PubMed: 24957906) »
    • Wang B  et al. p53 increases MHC class I expression by upregulating the endoplasmic reticulum aminopeptidase ERAP1. Nat Commun 4:2359 (2013). WB . Read more (PubMed: 23965983) »

    See all 3 Publications for this product

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    There are currently no Abreviews or Questions for ab69037.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"