製品の概要

  • 製品名Anti-ER81 antibody
    ER81 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ER81
  • アプリケーション適用あり: WB, ELISA, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Cow, Dog
  • 免疫原

    A synthetic peptide corresponding to the C-terminal of Human ER81 protein.

  • ポジティブ・コントロール
    • Tissue lysate derived from mouse brain.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
Flow Cyt Use a concentration of 2 - 5 µg/ml. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 2 - 5 µg/ml.

ターゲット情報

  • 機能Transcriptional activator that binds to DNA sequences containing the consensus pentanucleotide 5'-CGGA[AT]-3'.
  • 組織特異性Very highly expressed in brain, highly expressed in testis, lung and heart, moderately in spleen, small intestine, pancreas and colon, weakly in liver, prostate and thymus, very weakly in skeletal muscle, kidney and ovary and not in placenta and peripheral blood leukocytes.
  • 関連疾患Defects in ETV1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving ETV1 is found in patients with Erwing sarcoma. Translocation t(7;22)(p22;q12) with EWSR1.
  • 配列類似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • 翻訳後修飾Sumoylated.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp781L0674 antibody
    • ER81 antibody
    • ER81 protein antibody
    • ER81, mouse, homolog of antibody
    • ETS translocation variant 1 antibody
    • ets variant gene 1 antibody
    • Ets-related protein 81 antibody
    • Etsrp81 antibody
    • ETV 1 antibody
    • ETV1 antibody
    • ETV1_HUMAN antibody
    • MGC104699 antibody
    • MGC120533 antibody
    • MGC120534 antibody
    see all

Anti-ER81 antibody 画像

  • All lanes : Anti-ER81 antibody (ab81086) at 1/500 dilution

    Lane 1 : Tissue lysate derived from mouse brain
    Lane 2 : Tissue lysate derived from mouse brain, pre-incubated with immunizing peptide


    Predicted band size : 55 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab81086 (4µg/ml) staining ER81 in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining of seminal vesicles.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-ER81 antibody (ab81086) 使用論文

This product has been referenced in:
  • Breunig JJ  et al. Ets Factors Regulate Neural Stem Cell Depletion and Gliogenesis in Ras Pathway Glioma. Cell Rep 12:258-271 (2015). Mouse . Read more (PubMed: 26146073) »
  • Oka Y  et al. Thirst driving and suppressing signals encoded by distinct neural populations in the brain. Nature 520:349-52 (2015). IHC (PFA fixed) ; Mouse . Read more (PubMed: 25624099) »

See all 9 Publications for this product

Product Wall

Thank you for sending the protocol details and the images. I will send a replacement for the vial of the antibody that is giving the poor result but we only have one lot in stock, which is the same lot as the bad vial. I think it would be best to try a...

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Thank you for contacting us. Could you please provide the following information, and attach an image of the dirty blot, if available?

1. How much sample was loaded per lane of the gel? Is there any evidence from other blots of the same sampl...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (sarcoma)
Specification sarcoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6, 20 min, 100C
Permeabilization No
Username

Abcam user community

Verified customer

投稿 Dec 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (sarcoma)
Specification sarcoma
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TE-Buffer
Permeabilization Yes - DAKO Wash Buffer with tween 20
Username

Mr. Rudolf Jung

Verified customer

投稿 Feb 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"