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RabMAb

Anti-Epsin 1 抗体 [EPR3023] (ab75879)

製品の概要

  • 製品名
    Anti-Epsin 1 antibody [EPR3023]
    Epsin 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3023] to Epsin 1
  • アプリケーション
    適用あり: WB, ICC/IFmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Epsin 1 aa 450-550.
    Database link: Q9Y6I3

  • ポジティブ・コントロール
    • ICC/IF: SH-SY5Y cells WB: SH-SY5Y and HeLa cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR3023
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 58 kDa.
ICC/IF 1/50.

For unpurified, use 1/100 - 1/250.

  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能
      Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.
    • 配列類似性
      Belongs to the epsin family.
      Contains 1 ENTH (epsin N-terminal homology) domain.
      Contains 3 UIM (ubiquitin-interacting motif) repeats.
    • ドメイン
      The NPF repeat domain is involved in EPS15 binding.
      The DPW repeat domain is involved in AP2A2 and clathrin binding.
      The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction with the AP-2 complex subunit AP2B1.
    • 翻訳後修飾
      Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.
      Ubiquitinated.
    • 細胞内局在
      Cytoplasm. Cell membrane. Nucleus. Membrane > clathrin-coated pit. Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145.
    • Information by UniProt
    • 参照データベース
    • 別名
      • EH domain binding mitotic phosphoprotein antibody
      • EH domain-binding mitotic phosphoprotein antibody
      • EPN 1 antibody
      • Epn1 antibody
      • EPN1_HUMAN antibody
      • EPS 15 interacting protein 1 antibody
      • EPS-15-interacting protein 1 antibody
      • Epsin 1 antibody
      • Epsin-1 antibody
      see all

    画像

    • Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/5000 dilution (purified) + rat brain at 20 µg

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

      Predicted band size : 58 kDa
      Observed band size : 94 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/1000 dilution (purified)

      Lane 1 : 293T cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

      Predicted band size : 58 kDa
      Observed band size : 94 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/5000 dilution (purified) + mouse brain lysate at 20 µg

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

      Predicted band size : 58 kDa
      Observed band size : 94 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunofluorescence staining of HeLa cells with purified ab75879 at a working dilution of 1/50, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab75879 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • Unpurified ab75879, at a 1/100 dilution, staining Epsin 1 in SH-SY5Y cells by Immunofluorescence.

    • All lanes : Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/2000 dilution (unpurified)

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit-HRP at 1/1000 dilution
      Developed using the ECL technique

      Predicted band size : 58 kDa

    参考文献

    ab75879 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Thank you for contacting us. I have quickly checked and found that this protein indeed runs at 80-90 kDa. The 58 kDa is the molecular weight of unprocessed raw protein which is calculated by combining the weight of amino acids as advised in Swisspr...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (astrocytoma)
    Loading amount
    25 µg
    Specification
    astrocytoma
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
    Username

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    Verified customer

    投稿 Apr 09 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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