製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to EPS8
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide:

    SGVESFDEGSSH

    , corresponding to C terminal amino acids 811-822 of Human EPS8

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline. pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab38329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Preliminary experiments gave no signal but low background in A431 and Hela lysates at up to 1µg/ml.
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tested in Human Breast Carcinoma showing staining of cancer cells infiltrated in a breast lobe.

ターゲット情報

  • 機能
    Upon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets.
  • 組織特異性
    Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells.
  • 配列類似性
    Belongs to the EPS8 family.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • ドメイン
    The SH3 domain mediates interaction with SHB.
  • 翻訳後修飾
    Phosphorylated by several receptor tyrosine kinases.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Epidermal growth factor receptor kinase substrate 8 antibody
    • Epidermal growth factor receptor pathway substrate 8 antibody
    • EPS 8 antibody
    • EPS8 antibody
    • EPS8_HUMAN antibody
    see all

画像

  • ab38329 (5µg/ml) staining paraffin embedded Human Breast Carcinoma. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

参考文献

ab38329 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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