製品の概要

  • 製品名Anti-EPS15R antibody [EP1147Y]
    EPS15R 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1147Y] to EPS15R
  • アプリケーション適用あり: WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-terminus of human EPS15R was used as an immunogen

  • ポジティブ・コントロール
    • HeLa cell membrane or nuclear cell lysate Human breast carcinoma tissue
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50000. Detects a band of approximately 125 kDa (predicted molecular weight: 94 kDa).
IP 1/70.
ICC 1/50 - 1/100.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
  • 配列類似性Contains 1 EF-hand domain.
    Contains 3 EH domains.
  • 翻訳後修飾Phosphorylated on tyrosine residues by EGFR.
  • 細胞内局在Cell membrane. Nucleus. Membrane > coated pit. Localized to plasma membrane coated pits.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EP15R_HUMAN antibody
    • epidermal growth factor receptor pathway substrate 15- ike 1 antibody
    • Epidermal growth factor receptor substrate 15 like 1 antibody
    • Epidermal growth factor receptor substrate 15-like 1 antibody
    • epidermal growth factor receptor substrate EPS15R antibody
    • Eps15 related protein antibody
    • Eps15-related protein antibody
    • EPS15L1 antibody
    • Eps15R antibody
    see all

Anti-EPS15R antibody [EP1147Y] 画像

  • Anti-EPS15R antibody [EP1147Y] (ab53006) at 1/50000 dilution + HeLa cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP labelled at 1/2000 dilution

    Predicted band size : 94 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
  • ab53006, at a 1/50 dilution, staining Human EPS15R in Breast Carcinoma, using Immunohistochemsitry, Paraffin embedded tissue.

Anti-EPS15R antibody [EP1147Y] (ab53006) 使用論文

This product has been referenced in:
  • Liu Z & Zheng Y A requirement for epsin in mitotic membrane and spindle organization. J Cell Biol 186:473-80 (2009). Read more (PubMed: 19704019) »
  • Chen C & Zhuang X Epsin 1 is a cargo-specific adaptor for the clathrin-mediated endocytosis of the influenza virus. Proc Natl Acad Sci U S A 105:11790-5 (2008). Read more (PubMed: 18689690) »

See all 2 Publications for this product

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