Anti-Epoxide hydrolase 抗体 (ab96695)
Key features and details
- Rabbit polyclonal to Epoxide hydrolase
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Epoxide hydrolase antibody
Epoxide hydrolase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Epoxide hydrolase -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Pig -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-193 of Human Epoxide hydrolase (NP_000111).
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ポジティブ・コントロール
- WB: 293T, HeLa and HepG2 cell lysates ICC/IF: Hela cells IHC-P: SG xenograft
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96695の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 53 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. -
組織特異性
Found in liver. -
関連疾患
Note=In some populations, the high activity haplotype tyr113/his139 is overrepresented among women suffering from pregnancy-induced hypertension (pre-eclampsia) when compared with healthy controls.
Defects in EPHX1 are a cause of familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption. -
配列類似性
Belongs to the peptidase S33 family. -
細胞内局在
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 535293 Cow
- Entrez Gene: 2052 Human
- Entrez Gene: 13849 Mouse
- Entrez Gene: 397639 Pig
- Entrez Gene: 25315 Rat
- Omim: 132810 Human
- SwissProt: P07099 Human
- SwissProt: Q9D379 Mouse
see all -
別名
- EPHX 1 antibody
- EPHX antibody
- Ephx1 antibody
see all
画像
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All lanes : Anti-Epoxide hydrolase antibody (ab96695) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa
10% SDS PAGE -
ab96695, at a 1/200 dilution, staining Epoxide hydrolase in methanol fixed HeLa cells by Immunofluorescence analysis. Lower image costained with Hoerchst 33342.
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ab96695, at a 1/500 dilution, staining Epoxide hydrolase in paraffin embedded SG xenograft by Immunohistochemical analysis.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab96695 は 4 報の論文で使用されています。
- González-Alvarez ME et al. Obesity alters the ovarian proteomic response to zearalenone exposure†. Biol Reprod 105:278-289 (2021). PubMed: 33855340
- Cheng H et al. Emerging role of EPHX1 in chemoresistance of acute myeloid leukemia by regurlating drug-metabolizing enzymes and apoptotic signaling. Mol Carcinog N/A:N/A (2019). PubMed: 30644597
- Kumar M et al. Insulin activates intracellular transport of lipid droplets to release triglycerides from the liver. J Cell Biol 218:3697-3713 (2019). PubMed: 31604801
- O'Shaughnessy PJ et al. Maternal smoking and fetal sex significantly affect metabolic enzyme expression in the human fetal liver. J Clin Endocrinol Metab 96:2851-60 (2011). WB ; Human . PubMed: 21715529