製品の概要

  • 製品名Anti-EPO Receptor antibody
    EPO Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EPO Receptor
  • 特異性Detects total EPO Receptor levels.
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic non-phosphopeptide from human EPO Receptor, designed around the phosphorylation site of tyrosine 368 (DTYPLV)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab61162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ELISA 1/5000.

ターゲット情報

  • 機能Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.
    Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.
  • 組織特異性Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells.
  • 関連疾患Defects in EPOR are the cause of erythrocytosis familial type 1 (ECYT1) [MIM:133100]. ECYT1 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin and hematocrit, hypersensitivity of erythroid progenitors to erythropoietin, erythropoietin low serum levels, and no increase in platelets nor leukocytes. It has a relatively benign course and does not progress to leukemia.
  • 配列類似性Belongs to the type I cytokine receptor family. Type 1 subfamily.
    Contains 1 fibronectin type-III domain.
  • ドメインThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
    Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • 翻訳後修飾On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site.
    Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.
  • 細胞内局在Cell membrane and Secreted. Secreted and located to the cell surface.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EPO R antibody
    • EPO-R antibody
    • epor antibody
    • EPOR_HUMAN antibody
    • Erythropoietin receptor antibody
    • Erythropoietin receptor precursor antibody
    • MGC138358 antibody
    see all

Anti-EPO Receptor antibody 画像

  • ab166162 staining EPO Receptor in NIH3T3 cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 10% serim for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hour at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-EPO Receptor antibody (ab61162) at 1/500 dilution

    Lane 1 : Cos 7 cells treated with EPO (20U/ml, 15 mins)
    Lane 2 : Cos 7 cells treated with EPO (20U/ml, 15 mins) with immunizing peptide


    Predicted band size : 55 kDa
    Observed band size : 55 kDa

Anti-EPO Receptor antibody (ab61162) 使用論文

This product has been referenced in:
  • Suszynska M  et al. Expression of the erythropoietin receptor by germline-derived cells - further support for a potential developmental link between the germline and hematopoiesis. J Ovarian Res 7:66 (2014). ICC/IF . Read more (PubMed: 24982693) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH3T3)
Specification NIH3T3
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Mar 22 2013

Gracias por su respuesta.

Vuelvo a adjuntar el cuestionario al presente e-mail.

Espero su respuesta. Gracias por su colaboración.

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In or...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"