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Synthetic non-phosphopeptide from human EPO Receptor, designed around the phosphorylation site of tyrosine 368 (DTYPLV)
Our Abpromise guarantee covers the use of ab61162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
ab166162 staining EPO Receptor in NIH3T3 cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 10% serim for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hour at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.
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