EPO overexpression 293T lysate (whole cell) (ab94196)

製品の概要

  • 製品名EPO overexpression 293T lysate (whole cell)
  • 製品の詳細
    EPO overexpression lysate
  • 特記事項ab94196 is a 293T cell transfected lysate in which Human EPO has been transiently over-expressed using a pCMV-EPO plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 背景Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

アプリケーション

Our Abpromise guarantee covers the use of ab94196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

EPO overexpression 293T lysate (whole cell) 画像

  • ab94196 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-EPO antibody (ab66071) at 1/500 dilution

    Lane 1 : EPO overexpression 293T lysate (whole cell) (ab94196)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

EPO overexpression 293T lysate (whole cell) (ab94196) 使用論文

ab94196 has not yet been referenced specifically in any publications.

Product Wall

I think it best to heat the EPO positive lysate before loading it onto the gel.

This lysate is ready to use and can be loaded directly to the gel.

Typically, 15uL of the lysate is loaded per lane for western blotting assays.

Our R and D department suggests following these guidelines. For some proteins, it may b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"