製品の概要

  • 製品名Anti-EPO antibody [85]
    EPO 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [85] to EPO
  • アプリケーション適用あり: IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Purified CHO cell-expressed recombinant human EPO.

  • ポジティブ・コントロール
    • IHC-P: Human kidney FFPE tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab19485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 関連性Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
  • 細胞内局在Secreted
  • 参照データベース
  • 別名
    • EP antibody
    • EPO alpha antibody
    • EPO antibody
    • Epoetin antibody
    • Erythropoetin antibody
    • Erythropoietin precursor antibody
    • MVCD2 antibody
    see all

Anti-EPO antibody [85] 画像

  • IHC image of EPO staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19485, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-EPO antibody [85] (ab19485) 使用論文

ab19485 has not yet been referenced specifically in any publications.

Product Wall

Thank you for getting back to me. I can understand now the reason for selecting different EPO antibodies as free of charge replacements. This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vial...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"