製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to EPM2AIP1
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Cynomolgus monkey
  • 免疫原

    Synthetic peptide:

    TRNQHTLSQPLTDEH

    by a Cysteine residue linker, corresponding to internal sequence amino acids 565-579 of Human EPM2AIP1 (NP_055620.1).

  • ポジティブ・コントロール
    • HEK293 lysate overexpressing Human EPM2AIP1.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab112495 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab112495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 70 kDa.
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

画像

  • ab112495 (5µg/ml) shows staining of EPM2AIP1 in paraffin embedded Human Heart following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows regular staining of sarcoplasmic reticulum in the cardiac fibers.

  • Lane 1 : Anti-EPM2AIP1 antibody (ab112495) at 1 µg/ml
    Lane 2 : Anti-EPM2AIP1 antibody (ab112495) at 1 mg/ml
    Lane 3 : anti-DYKDDDDK Tag antibody at 1/1000 dilution

    Lane 1 : HEK293 lysate overexpressing Human EPM2AIP1 with DYKDDDDK tag
    Lane 2 : Mock-transfected HEK293
    Lane 3 : HEK293 lysate overexpressing Human EPM2AIP1 with DYKDDDDK tag

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 70 kDa
    Primary incubations were for 1 hour. Detected by chemiluminescence.

参考文献

ab112495 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab112495.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録