Anti-EPLIN 抗体 (ab50196)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to EPLIN
  • 特異性
    This antibody reacts with EPLIN
  • アプリケーション
    適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow, Dog, Pig
  • 免疫原

    A region within synthetic peptide: GVLAASMEAK ASSQQEKEDK PAETKKLRIA WPPPTELGSS GSALEEGIKM, corresponding to amino acids 502-551 of Human EPLIN

  • ポジティブ・コントロール
    • Placenta lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa).Can be blocked with Human EPLIN peptide (ab111676). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/12500.

ターゲット情報

  • 機能
    Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments.
  • 組織特異性
    Highly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart. Also detected in lung, liver, brain, skeletal muscle, thymus, testis and intestine. Not detected in leukocytes. Isoform Beta expressed generally at very low levels. Isoform Alpha abundant in epithelial cells from mammary gland, prostate and in normal oral keratinocytes. Low levels in aortic endothelial cells and dermal fibroblasts. Not detectable in myocardium.
  • 配列類似性
    Contains 1 LIM zinc-binding domain.
  • ドメイン
    Contains at least 2 actin-binding domains, one on each side of the LIM domain. Both domains bind actin monomers and filaments. The C-terminal domain binds filaments more efficiently than the N-terminus.
  • 細胞内局在
    Cytoplasm. Cell junction > focal adhesion. Cytoplasm > cytoskeleton. This cytoskeletal protein co-localizes with actin stress fibers and focal adhesion plaques.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1110021C24Rik antibody
    • Epithelial protein lost in neoplasm antibody
    • Epithelial protein lost in neoplasm beta antibody
    • EPLIN antibody
    • FLJ38853 antibody
    • LIM domain and actin binding 1 antibody
    • LIM domain and actin binding protein 1 antibody
    • LIM domain and actin-binding protein 1 antibody
    • LIMA1 antibody
    • LIMA1_HUMAN antibody
    • MGC131726 antibody
    • SREBP3 antibody
    • Sterol regulatory element binding protein 3 antibody
    see all

画像

  • Anti-EPLIN antibody (ab50196) at 2 µg/ml + Placental lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 85 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

参考文献

ab50196 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your email. I have checked the protocol you have kindly sent and would like to make few suggestions that I am sure will help to get staining at right location; - Use 5% BSA blocking solution for 1-1.5 hours. - Try using more dil...

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Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I am atta...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Kidney)
Specification
Kidney
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C
Username

晴子 鶴見

Verified customer

投稿 Nov 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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