製品の概要

  • 製品名Anti-EPHX2 antibody
    EPHX2 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to EPHX2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length EPHX2 protein (Human)

  • ポジティブ・コントロール
    • EPHX2 transfected 293T cell lysate and human kidney tissue lysate

アプリケーション

Our Abpromise guarantee covers the use of ab67788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 63 kDa.

ターゲット情報

  • 機能Acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. Has low phosphatase activity.
  • 配列類似性Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.
  • ドメインThe N-terminal domain has phosphatase activity. The C-terminal domain has epoxide hydrolase activity.
  • 翻訳後修飾The N-terminus is blocked.
    The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation.
  • 細胞内局在Cytoplasm. Peroxisome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Bifunctional epoxide hydrolase 2 antibody
    • CEH antibody
    • Cytosolic epoxide hydrolase antibody
    • EPHX2 antibody
    • Epoxide hydratase antibody
    • Epoxide hydrolase 2 antibody
    • Epoxide hydrolase 2 cytoplasmic antibody
    • epoxide hydrolase 2, cytosolic antibody
    • Epoxide hydrolase soluble antibody
    • HYES_HUMAN antibody
    • SEH antibody
    • Soluble epoxide hydrolase antibody
    see all

Anti-EPHX2 antibody 画像

  • Anti-EPHX2 antibody (ab67788) at 1/500 dilution + human kidney tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 63 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-EPHX2 antibody (ab67788) at 1/500 dilution

    Lane 1 : EPHX2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 63 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

Anti-EPHX2 antibody (ab67788) 使用論文

ab67788 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (Recombinant protein)
Loading amount 0.02 µg
Specification Recombinant protein
Gel Running Conditions Reduced Denaturing (5-20% gradient)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Dec 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"