Anti-Ephrin B (phospho Y331) 抗体 (ab32586)

製品の概要

  • 製品名
    Anti-Ephrin B (phospho Y331) antibody
    Ephrin B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ephrin B (phospho Y331)
  • 特異性
    ab32586 detects Phospho-EphrinB (Tyr331).
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human, Xenopus laevis
  • 免疫原

    Synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphorylated Tyrosine 331 of chicken Ephrin B

  • ポジティブ・コントロール
    • Rat testes tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Detects a band of approximately 46 kDa (predicted molecular weight: 38 kDa).

ターゲット情報

  • 関連性
    Ephrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase.
  • 細胞内局在
    Membrane; single pass type I membrane protein.
  • 参照データベース
  • 別名
    • CEK5 ligand antibody
    • EFNB1 antibody
    • ELK L antibody
    • ELK ligand antibody
    • ELKL antibody
    • EPH related receptor tyrosine kinase ligand 2 antibody
    • Ephrin B1 antibody
    • Ephrin B2 antibody
    • LERK2 antibody
    see all

画像

  • All lanes : Anti-Ephrin B (phospho Y331) antibody (ab32586)

    Lane 1 : rat testes lysate
    Lane 2 : rat testes lysate with previous incubation with 1200 units of lambda-phosphatase for 30min


    Predicted band size : 38 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab32586 has not yet been referenced specifically in any publications.

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