製品の概要

  • 製品名Anti-Ephrin A5 antibody
    Ephrin A5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ephrin A5
  • 特異性ab70114 detects endogenous levels of total Ephrin A5 protein.
  • アプリケーション適用あり: Flow Cyt, IHC-Fr, WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of human Ephrin A5.

  • ポジティブ・コントロール
    • A549 cells, HeLa cell extracts and human brain tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. PubMed: 22071104ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration. PubMed: 21628570
WB 1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 26 kDa).
ELISA 1/40000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

ターゲット情報

Anti-Ephrin A5 antibody 画像

  • ab70114 staining anti-Ephrin A5 in wild-type and knockout mouse inferior colliculus/cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 15 minutes at 20°C; antigen retrieval was by heat mediation with citric acid. Samples were incubated with primary antibody (1/200 in 1 % TBS/BSA buffer) for 2 hours at 20°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab70114 at 1/500 dilution staining Ephrin A5 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • ab70114 at 1/50 dilution staining Ephrin A5 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide
  • All lanes : Anti-Ephrin A5 antibody (ab70114) at 1/500 dilution

    Lane 1 : HeLa cell extracts
    Lane 2 : HeLa cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 26 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)

Anti-Ephrin A5 antibody (ab70114) 使用論文

This product has been referenced in:
  • Son AI  et al. Formation of persistent hyperplastic primary vitreous in ephrin-A5-/- mice. Invest Ophthalmol Vis Sci 55:1594-606 (2014). Read more (PubMed: 24550361) »
  • Brennaman LH  et al. Polysialylated NCAM and EphrinA/EphA Regulate Synaptic Development of GABAergic Interneurons in Prefrontal Cortex. Cereb Cortex : (2012). Mouse . Read more (PubMed: 22275477) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric Acid
Sample Mouse Tissue sections (inferior colliculus/cerebellum)
Specification inferior colliculus/cerebellum
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 04 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brainstem)
Specification Brainstem
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
Username

Mr. Carl Hobbs

Verified customer

投稿 Apr 05 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Cerebellum)
Specification Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Mar 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (flag-tagged mouse ephrinA5 expressed in CHO cells)
Specification flag-tagged mouse ephrinA5 expressed in CHO cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jan 03 2013

Thank you very much for providing that information. It makes understanding the problems you have been experiencing with the antibody much easier.

I am sorry that you have not obtained the expected results with this antibody. I'd like to ask a...

Read More
Application Flow Cytometry
Sample Human Cell (many normal and tumor cell lines)
Specification many normal and tumor cell lines
Permeabilization No
Username

Abcam user community

Verified customer

投稿 Jul 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"