製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Ephexin-1
  • アプリケーション
    適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 1-133 of Human Ephexin-1.

  • ポジティブ・コントロール
    • Human cerebral cortex tissue; A431 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab121274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

ターゲット情報

  • 機能
    Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension.
  • 組織特異性
    Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung.
  • 配列類似性
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • ドメイン
    The DH domain and the PH domain are both required to mediate interaction with EPHA4.
  • 翻訳後修飾
    Src-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA.
  • 細胞内局在
    Cytoplasm. Membrane. Cell projection > growth cone. Associated with membranes. Localizes to axonal growth cones.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARHGEF27 antibody
    • Eph receptor-interacting exchange protein antibody
    • Eph-interacting exchange protein antibody
    • EPHEXIN antibody
    • Ephexin-1 antibody
    • Guanine nucleotide exchange factor, neuronal antibody
    • Neuronal guanine nucleotide exchange factor antibody
    • Ngef antibody
    • NGEF_HUMAN antibody
    see all

画像

  • ab121274 at 1/100 dilution staining Ephexin-1 in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
  • ab121274 at 4 µg/ml staining Ephexin-1 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

参考文献

ab121274 has not yet been referenced specifically in any publications.

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