Anti-Eph receptor B4/HTK 抗体 [RM0056-6A14] (ab73259)
Key features and details
- Rat monoclonal [RM0056-6A14] to Eph receptor B4/HTK
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG1
製品の概要
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製品名
Anti-Eph receptor B4/HTK antibody [RM0056-6A14]
Eph receptor B4/HTK 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [RM0056-6A14] to Eph receptor B4/HTK -
由来種
Rat -
特異性
ab73259 is specific for mouse Eph receptor B4/HTK. It does not cross-react with recombinant mouse EphA2, EphA3, EphA4, EphA6, EphA7 and EphB6
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アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment corresponding to Mouse Eph receptor B4/HTK (extracellular).
Database link: P54761 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
ab73259 was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with recombinant mouse Eph receptor B4/HTK extracellular domain). -
ポリ/モノ
モノクローナル -
クローン名
RM0056-6A14 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab73259の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
1/100 - 1/200.
Perform enzymatic antigen retrieval with 20 ug/ml proteinase K before commencing with IHC protocol. |
特記事項 |
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IHC-P
1/100 - 1/200. Perform enzymatic antigen retrieval with 20 ug/ml proteinase K before commencing with IHC protocol. |
ターゲット情報
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機能
Receptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development. -
組織特異性
Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
翻訳後修飾
Autophosphorylated. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 13846 Mouse
- SwissProt: P54761 Mouse
- Unigene: 34533 Mouse
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別名
- Ephb4 antibody
- EPHB4_HUMAN antibody
- Ephrin receptor EphB4 antibody
see all
画像
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ab73259 at 1/200 dilution, staining Eph receptor B4/HTK in LPS treated mouse kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
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ab73259 staining the Eph receptor B4/HTK in Mouse lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was not performed. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-rat polyclonal (1/200) was used as the secondary antibody.
データシートおよび資料
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Datasheet download
参考文献 (4)
ab73259 は 4 報の論文で使用されています。
- Wang Y et al. Loading of erythropoietin on biphasic calcium phosphate bioceramics promotes osteogenesis and angiogenesis by regulating EphB4/EphrinB2 molecules. J Mater Sci Mater Med 33:19 (2022). PubMed: 35072831
- Sagar V et al. EPHB4 inhibition activates ER stress to promote immunogenic cell death of prostate cancer cells. Cell Death Dis 10:801 (2019). PubMed: 31641103
- Munro DAD et al. Refuting the hypothesis that semaphorin-3f/neuropilin-2 exclude blood vessels from the cap mesenchyme in the developing kidney. Dev Dyn 246:1047-1056 (2017). PubMed: 28929539
- Chen II et al. EphrinB2 regulates the emergence of a hemogenic endothelium from the aorta. Sci Rep 6:27195 (2016). ICC/IF ; Mouse . PubMed: 27250641