Anti-Eph receptor B4 抗体 (ab93902)

製品の概要

  • 製品名Anti-Eph receptor B4 antibody
    Eph receptor B4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Eph receptor B4
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow, Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human Eph receptor B4.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both Human placenta tissue lysate and K562 whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 108 kDa).

Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product.

ターゲット情報

  • 機能Receptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development.
  • 組織特異性Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • 翻訳後修飾Autophosphorylated.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Ephb4 antibody
    • EPHB4_HUMAN antibody
    • Ephrin receptor EphB4 antibody
    • Ephrin type-B receptor 4 antibody
    • Hepatoma Transmembrane Kinase antibody
    • HTK antibody
    • MYK 1 antibody
    • MYK1 antibody
    • TYRO 11 antibody
    • TYRO11 antibody
    • Tyrosine-protein kinase receptor HTK antibody
    • Tyrosine-protein kinase TYRO11 antibody
    see all

Anti-Eph receptor B4 antibody 画像

  • All lanes : Anti-Eph receptor B4 antibody (ab93902) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 108 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

    The predicted molecular weight of Eph receptor B4 is 108 kDa (SwissProt), however we expect to observe a banding pattern at 125 kDa. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

Anti-Eph receptor B4 antibody (ab93902) 使用論文

ab93902 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50000 cells
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (A549, HEK293)
Specification A549, HEK293
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Apr 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"