製品の概要

  • 製品名Anti-Eph receptor B4 antibody
    Eph receptor B4 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Eph receptor B4
  • アプリケーション適用あり: ELISA, Dot blot, Inhibition Assaymore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Purified recombinant mouse EphB4 extracellular domain expressed in mouse NSO cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
Dot blot
Inhibition Assay
  • 追加情報Dot: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant mouse EphB4 is approximately 5 ng/lane under non-reducing and reducing conditions.
    ELISA: Use at a concentration of 0.5 - 1.0 µg/ml, detection limit 1.5 ng/well.
    Inhib: Use at a concentration of 0.5 - 2 µg/ml. For neutralization of receptor-ligand interaction, approximately 0.5-2 µg/ml of the antibody will block 50% of the binding of recombinant mouse ephrin-B2/ (5 ng/ml) to immobilized recombinant mouse EphB4/Fc chimera (100 µl of a 2 µg/ml solution coated in each well).

    The recombinant mouse EphB4 used for the testing of this antibody consists of the extracellular domain of mouse EphB4 (amino acids 1-539) fused by means of a polypeptide linker to the Fc portion of human IgG1 that is histidine-tagged at the C-terminus. N-terminal sequencing indicates that recombinant mouse EphB4/Fc has Leu 16 at the amino terminus. The calculated molecular mass of the reduced protein is approximately 85.2 kDa, but as a result of glycosylation, recombinant EphB4/Fc migrates as an approximately 110 kDa protein under reducing conditions in SDS-PAGE. EphB4 binds to ephrin-B1 and ephrin-B2. Human and mouse EphB4 extracellular domains share approximately 88% homology.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Receptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development.
    • 組織特異性Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells.
    • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 1 protein kinase domain.
      Contains 1 SAM (sterile alpha motif) domain.
    • 翻訳後修飾Autophosphorylated.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Ephb4 antibody
      • EPHB4_HUMAN antibody
      • Ephrin receptor EphB4 antibody
      • Ephrin type-B receptor 4 antibody
      • Hepatoma Transmembrane Kinase antibody
      • HTK antibody
      • MYK 1 antibody
      • MYK1 antibody
      • TYRO 11 antibody
      • TYRO11 antibody
      • Tyrosine-protein kinase receptor HTK antibody
      • Tyrosine-protein kinase TYRO11 antibody
      see all

    Anti-Eph receptor B4 antibody (ab10618) 使用論文

    ab10618 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"