製品の概要

  • 製品名
    Anti-Eph receptor B2 antibody
    Eph receptor B2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Eph receptor B2
  • アプリケーション
    適用あり: ICC/IF, IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids 118-133 of Human Eph B2.

  • ポジティブ・コントロール
    • Mouse heart tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
IHC-P
ELISA
WB
  • 追加情報
    ELISA: 1/1000.
    WB: 1/100 - 1/500. Detects a band of approximately 116 kDa (predicted molecular weight: 118 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Receptor for members of the ephrin-B family. Phosphorylates ARHGEF15, leading to its ubiquitination and degradation by the proteasome which promotes EFNB1-dependent synapse formation. Can function in aspects of retinal ganglion cell axon guidance to the optic disk even when lacking its tyrosine kinase domain. Acts as a tumor suppressor.
    • 組織特異性
      Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain.
    • 関連疾患
      Defects in EPHB2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. Note=EPHB2 mutations have been found in a prostate cancer cell line derived from a brain metastasis.
    • 配列類似性
      Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 1 protein kinase domain.
      Contains 1 SAM (sterile alpha motif) domain.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • cek5 antibody
      • Developmentally regulated EPH related tyrosine kinase antibody
      • DRT antibody
      • EK5 antibody
      • ELK related protein tyrosine kinase antibody
      • Eph receptor B2 antibody
      • EPH tyrosine kinase 3 antibody
      • EPH-like kinase 5 antibody
      • EPHB2 antibody
      • EPHB2_HUMAN antibody
      • Ephrin type B receptor 2 antibody
      • Ephrin type-B receptor 2 antibody
      • EPHT 3 antibody
      • ERK antibody
      • ETECK antibody
      • hEK5 antibody
      • Nuk antibody
      • Prkm 5 antibody
      • Receptor protein tyrosine kinase HEK 5 antibody
      • Renal carcinoma antigen NY-REN-47 antibody
      • Sek 3 antibody
      • Tyro 5 antibody
      • Tyrosine protein kinase receptor CEK 5 antibody
      • Tyrosine protein kinase receptor EPH 3 antibody
      • Tyrosine protein kinase receptor QEK 5 antibody
      • Tyrosine-protein kinase receptor EPH-3 antibody
      • Tyrosine-protein kinase TYRO5 antibody
      see all

    画像

    • Anti-Eph receptor B2 antibody (ab5418) at 1/100 dilution + mouse heart tissue lysate at 0.01 µg
      Developed using the ECL technique

      Predicted band size : 118 kDa
      Observed band size : 118 kDa
      Additional bands at : 48 kDa (possible isoform).
    • Human cancer tissue stained with ab5418 followed by peroxidase-conjugated secondary antibody and AEC.
    • ICC/IF image of ab5418 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5418, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    参考文献

    This product has been referenced in:
    • Zhang C  et al. Growth of tyrosine kinase inhibitor-resistant Philadelphia-positive acute lymphoblastic leukemia: Role of bone marrow stromal cells. Oncol Lett 13:2059-2070 (2017). WB . Read more (PubMed: 28454362) »
    • Chakraborty S  et al. Kaposi's sarcoma-associated herpesvirus interacts with EphrinA2 receptor to amplify signaling essential for productive infection. Proc Natl Acad Sci U S A 109:E1163-72 (2012). WB, ICC/IF ; Human . Read more (PubMed: 22509030) »

    See all 4 Publications for this product

    レビューと Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SW480/SW620/LoVo/K9/Jurkate)
    Loading amount
    8 µg
    Specification
    SW480/SW620/LoVo/K9/Jurkate
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Dr. Tao Fu

    Verified customer

    投稿 Dec 17 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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