Anti-Eph receptor A2 抗体 [RM-0051-8F21] (ab73254)
Key features and details
- Rat monoclonal [RM-0051-8F21] to Eph receptor A2
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Eph receptor A2 antibody [RM-0051-8F21]
Eph receptor A2 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [RM-0051-8F21] to Eph receptor A2 -
由来種
Rat -
特異性
ab73254 is specific for Eph receptor A2. It does not cross react with recombinant mouse Eph receptors A3, 4, 6, 7, 8, and Eph receptors B1, 2, 3, 4, or 6. -
アプリケーション
適用あり: IHC-P, Flow Cytmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant mouse EphA2 extracellular domain
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab73254 was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with recombinant mouse EphA2 extracellular domain. The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and lyophilized from a 0.2 µm filtered solution. -
ポリ/モノ
モノクローナル -
クローン名
RM-0051-8F21 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab73254の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100 - 1/200.
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Flow Cyt | (1) |
Use 1µg for 106 cells.
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IHC-P
1/100 - 1/200. |
Flow Cyt
Use 1µg for 106 cells. ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 and -A5. Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Induces apoptosis in a p53/TP53-independent, caspase-8-dependent manner. -
組織特異性
Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary. -
関連疾患
Genetic variations in EPHA2 are the cause of susceptibility to cataract cortical age-related type 2 (ARCC2) [MIM:613020]. A developmental punctate opacity common in the cortex and present in most lenses. The cataract is white or cerulean, increases in number with age, but rarely affects vision.
Defects in EPHA2 are the cause of cataract posterior polar type 1 (CTPP1) [MIM:116600]. A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
翻訳後修飾
Activated by EFNA1 via tyrosine phosphorylation. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit. These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and endothelial cell migration. They also play a critical role in transducing EPHA2 signaling in vascular endothelial cells during tumor angiogenesis. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1969 Human
- Entrez Gene: 13836 Mouse
- Omim: 176946 Human
- SwissProt: P29317 Human
- SwissProt: Q03145 Mouse
- Unigene: 171596 Human
- Unigene: 2581 Mouse
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別名
- ARCC2 antibody
- AW545284 antibody
- CTPA antibody
see all
画像
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ab73254 at 1/200 dilution, staining Eph receptor A2 in mouse kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
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Overlay histogram showing A431 cells stained with ab73254 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73254, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was goat anti-rat DyLigt 488 (IgG H+L) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG1 [RTK2071] (ab18412, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (4)
ab73254 は 4 報の論文で使用されています。
- Jeong MG et al. Analysis of transient membrane protein interactions by single-molecule diffusional mobility shift assay. Exp Mol Med 53:291-299 (2021). PubMed: 33603128
- Zong S et al. A Chinese Herbal Formula Suppresses Colorectal Cancer Migration and Vasculogenic Mimicry Through ROS/HIF-1a/MMP2 Pathway in Hypoxic Microenvironment. Front Pharmacol 11:705 (2020). PubMed: 32499699
- Wei Q et al. EphA2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer. Mol Med Rep 22:2941-2947 (2020). PubMed: 32945400
- Ema A et al. Immunohistochemical analysis of RTKs expression identified HER3 as a prognostic indicator of gastric cancer. Cancer Sci 105:1591-600 (2014). PubMed: 25455899