Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) 抗体 [EPR5684] (ab124881)

製品の概要

  • 製品名Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684]
    EPH A3+A4+A5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5684] to EPH A3+A4+A5 (phospho Y779 + Y779 + Y833)
  • 特異性This antibody only detects EPH A3 phosphorylated at Tyrosine 779, EPH A4 phosphorylated at Tyrosine 779, and EPH A5 phosphorylated at Tyrosine 833.
  • アプリケーション適用あり: WBmore details
    適用なし: ICC/IF or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human EPH A3+A4+A5.

  • ポジティブ・コントロール
    • HeLa cell lysate (untreated/treated with Sodium Orthovanadate). Prepare 500mM stock Sodium Orthovanadate in PBS. When ready to treat cells add 400µl 500mM Sodium Orthovanadate to 1560µl H2O and then 40µl H2O2, vortex and incubate at RT for 5-10min. Serum-starve cells overnight, and treat with the freshly made Pervanadate for 20min at 37°C.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab124881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000. Detects a band of approximately 133 kDa (predicted molecular weight: 110, 115 kDa).

For unpurified, use 1/1000 - 1/10000.

  • 追加情報Is unsuitable for ICC/IF or IHC-P.
  • ターゲット情報

    • 関連性Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal andhippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. Beside its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion.
    • 細胞内局在Cell Membrane
    • 参照データベース
    • 別名
      • CEK7 antibody
      • EHK 1 antibody
      • EHK1 antibody
      • EK4 antibody
      • EK7 antibody
      • EK8 antibody
      • EPH homology kinase 1 antibody
      • EPH like kinase 4 antibody
      • EPH like kinase 7 antibody
      • EPH like kinase 8 antibody
      • Eph like tyrosine kinase 1 antibody
      • EPH receptor A3 antibody
      • EPH receptor A4 antibody
      • EPH receptor A5 antibody
      • EPHA3 antibody
      • EPHA4 antibody
      • EPHA5 antibody
      • Ephrin type A receptor 3 antibody
      • Ephrin type A receptor 4 antibody
      • Ephrin type A receptor 5 antibody
      • ETK antibody
      • ETK1 antibody
      • HEK antibody
      • HEK4 antibody
      • HEK7 antibody
      • HEK8 antibody
      • Human embryo kinase 1 antibody
      • OTTHUMP00000159594 antibody
      • OTTHUMP00000159595 antibody
      • OTTHUMP00000164185 antibody
      • OTTHUMP00000204491 antibody
      • OTTHUMP00000213903 antibody
      • OTTHUMP00000213904 antibody
      • OTTHUMP00000213905 antibody
      • OTTHUMP00000218917 antibody
      • OTTHUMP00000223029 antibody
      • Receptor protein tyrosine kinase HEK7 antibody
      • Receptor protein tyrosine kinase HEK8 antibody
      • SEK antibody
      • TYRO1 antibody
      • TYRO1 protein tyrosine kinase antibody
      • TYRO4 antibody
      • TYRO4 protein tyrosine kinase antibody
      • Tyrosine protein kinase receptor EHK 1 antibody
      • Tyrosine protein kinase receptor ETK1 antibody
      • Tyrosine protein kinase receptor SEK antibody
      • Tyrosine protein kinase TYRO1 antibody
      see all

    Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] 画像

    • All lanes : Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (ab124881) at 1/10000 dilution (purified)

      Lane 1 : Untreated HEK293 cell lysate
      Lane 2 : HEK293 cell lysate treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 110, 115 kDa
      Observed band size : 133 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (ab124881) at 1/1000 dilution (unpurified)

      Lane 1 : HeLa cell lysate untreated
      Lane 2 : HeLa cell lysate treated with Sodium Orthovanadate. See suggested positive control for more treatment information.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 110, 115 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (ab124881) 使用論文

    ab124881 has not yet been referenced specifically in any publications.

    Product Wall

    Sodium orthovandate treatment of HeLa cells is the suggested positive control. Please treat them as described below:

    Prepare 500mM stock Sodium Orthovanadate in PBS. When ready to treat cells add 400µl 500mM Sodium Orthovanadate to 156...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"