製品の概要

  • 製品名Anti-EPCR/CD201 antibody [RCR-252]
    EPCR/CD201 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [RCR-252] to EPCR/CD201
  • アプリケーション適用あり: Inhibition Assay, Flow Cyt, IP, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Tail base Human EPCR/CD201-positive RE-1 cells

  • エピトープAntibody RCR-252 stains the extracellular domain of human EPCR.
  • ポジティブ・コントロール
    • RE-1 (rat 3Y1 fibroblasts stably transfected with human EPCR).
  • 特記事項


    Ablates APC binding to EPCR/CD201 thus blocking APC-mediated signalling.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Inhibition Assay Use a concentration of 10 - 25 µg/ml. Antibody RCR-252 functions as blocking antbody. It ablates APC binding to EPCR and blocks APC-mediated signaling.
Flow Cyt 1/50. ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB 1/50. Use under non reducing condition.

ターゲット情報

  • 機能Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.
  • 組織特異性Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.
  • 翻訳後修飾N-glycosylated.
    A soluble form exists; probably released by a metalloprotease. Seems to have the same activity as the membrane-bound form.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activated protein C receptor antibody
    • APC receptor antibody
    • APCR antibody
    • bA42O4.2 antibody
    • CCCA antibody
    • CCD41 antibody
    • CD201 antibody
    • CD201 antigen antibody
    • cell cycle, centrosome-associated protein antibody
    • Cell cycle, centrosome-associated protein antibody
    • centrocyclin antibody
    • Endothelial cell protein C receptor antibody
    • Endothelial protein C receptor antibody
    • EPCR antibody
    • EPCR_HUMAN antibody
    • MGC23024 antibody
    • PROCR antibody
    • Protein C receptor, endothelial antibody
    see all

Anti-EPCR/CD201 antibody [RCR-252] (ab81712) 使用論文

This product has been referenced in:
  • Dömötör E  et al. Activated protein C alters cytosolic calcium flux in human brain endothelium via binding to endothelial protein C receptor and activation of protease activated receptor-1. Blood 101:4797-801 (2003). Inhibition Assay ; Human . Read more (PubMed: 12586611) »
  • Sturn DH  et al. Expression and function of the endothelial protein C receptor in human neutrophils. Blood 102:1499-505 (2003). Flow Cyt ; Human . Read more (PubMed: 12714492) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry and interest in our products.

As the datasheet indicates this product (ab81712: Anti-EPCR/CD201 antibody) has been tested and characterized in Inhibition Assay, Flow Cyt, IP, WB so we can offer our guarantee for the...

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Thanks for your email. I'm very sorry that even the replacement antibody still does not appear to be working as expected.

To help improve your results, Iam happy to provide a replacement of any primary antibody, credit or refund. Please let m...

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Thank you for your reply.

While we do not currently have in house testing data using ab81712 in Flow Cytometry on neutrophils, this clone has been used on human neutrophils in the following paper:

http://bloodjournal.hematologylibra...

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Thank you for contacting us. I am sorry that this antibody is not giving signal in Flow Cytometry on your human samples. Based on your protocol, my only recommendation would be to increase your primary and secondary antibody incubation times to at leas...

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Thank you for contacting Abcam.

Below is a suitable secondary to use with ab81712 in immunocytochemistry:

http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-HRP-pre-adsorbed-ab98425.html



However,...

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Sure, this should be fine too. ab6519 will react specifically with the Fab fragment of rat IgG.

I hope this is helpful. Please contact me again if you have any further questions.

Thanks for your enquiry.

ab81712 is a rat monoclonal, IgG1. http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-PE-pre-adsorbed-ab98392.htmlis a goat anti-rat- PE conjugated secondary antibody, catalog number ab98392. It wi...

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Thank you for contacting Abcam.

For ab81712, I would recommend that you use ab37361 as an isotype control:

http://www.abcam.com/Rat-IgG-Isotype-Control-ab37361.html



If there is anything else I can help you with,...

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Thank you for your phone call.

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I am very pleased to hear you would like to accept our offer and test ab81712 in dog and ICC. This code will give you: 1 free PRIMARY ANTIBODYbefore the ex...

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Thanks for your call yesterday and for your patience while I have been in touch with the lab about ab81712. The production lab did not test this antibody in-house in Western blot, and the Western blot information originates from the paper that I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"