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LoVo cell line preparation (Human).
Cross reacts with breast carcinomas, kidney tubules, tonsil crypt epithelium and cells in some serious effusions. Useful in characterisation of all epithelial cells.
Our Abpromise guarantee covers the use of ab20160 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. Used at a dilution of 1/250 for 1 hr - see Abreview for further information.|
|Flow Cyt||Use 1µg for 106 cells. (methanol fixed cells)
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab20160 at a 1/250 dilution staining human non small cell lung cancer cells (H358 cells) by immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using an Alexa-Fluor® 546 conjugated goat anti mouse antibody.
This image is courtesy of an Abreview submitted by an anonymous researcher on 30 January 2006.