製品の概要

  • 製品名Anti-EPC1 antibody
    EPC1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EPC1
  • アプリケーション適用あり: ICC/IF, ICC, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human EPC1.

    (Peptide available as ab24396.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HeLa, Hek293, U2OS
  • 特記事項From initial efforts, this antibody does not appear to work convincingly in Western Blot. Collaborative data has also found that this ab does not work in WB even with an overexpressed flagged form of EPC1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution.
ICC 1/50.
WB 1/1000.

ターゲット情報

  • 機能Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage.
  • 配列類似性Belongs to the enhancer of polycomb family.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp781P2312 antibody
    • Enhancer of polycomb 1 antibody
    • Enhancer of polycomb homolog 1 (Drosophila) antibody
    • Enhancer of polycomb homolog 1 antibody
    • EPC1 antibody
    • EPC1_HUMAN antibody
    • Epl 1 antibody
    • Epl1 antibody
    • OTTHUMP00000046260 antibody
    see all

Anti-EPC1 antibody 画像

  • Specificity of ab5514 against EPC1

    A and B: Detection using indirest immunofluorescence of the signal corresponding to EPC1 in EPC1 in 293 Cells were fixed using 4% formaldehyde blocked with PBS containing 3% milk and 0.5% Triton x-100 (sigma) incubated for 1 hour at 37 C with ab5514 at a 1/50 dilution. The cells were then washed three times and incubated for 1 hour at 37 C with a donkey anti-rabbit antibody coupled with Alexa Fluor 488 (Molecular probes) at a 1/500 dilution, washed three times, stained with DAPI and mounted with Vectasheild(Abcyss). A: Acquisition on a CCD camera B: Confocal microscopy (3um stack along Z axis).

    C.

  • Verification of the specificity of ab5514 against EPC1

    The siRNA that extinguishes EPC1 antibody-associated signal were independently validated using quantitative RT-PCR.

    D: Detection using indirect immunofluorescence of the signal corresponding to EPC1 in 293 cells. Cells were transfected with a control shRNA directed against luciferase (Siluc, left) or two siRNAs directed againstEPC1 (siR1EPC1, middle, and siR2EPC1, right). 24 hours after transfection cells were fixed, blocked, incubated for 1 hr at 37 C with ab5514 at a 1/50 dilution. The cells were then washed three times and incubated for 1 hour at 37 C with a donkey anti-rabbit antibody coupled with Alexa Fluor 488 (Molecular probes) at a 1/500 dilution, washed three times, stained with DAPI and mounted with Vectasheild(Abcyss). E: Efficiency o

  • All lanes : Anti-EPC1 antibody (ab5514) at 2 µg/ml

    Lane 1 : Whole cell lysate from HEK293 cell line
    Lane 2 : Whole cell lysate from HeLa cell line
    Lane 3 : Whole cell lysate from U20S cell line

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP-conjugated pig anti rabbit Ig

    Performed under reducing conditions.

    Exposure time : 15 minutes

    This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.

    Detection method: Supersignal West Dura. Note: There are 3 characterised isoforms of EPC1 which give rise to proteins of 93 kDa, 90 kDa and 85 kDa (Swissprot). This antibody detects a species of ~85 kDa in all lysates, including HEK293 cells which are known to express EPC1. The clean blots, together with the IF data (see datasheet), suggests that this antibody is specific to EPC1 and will work in western blotting.

    See Abreview

Anti-EPC1 antibody (ab5514) 使用論文

ab5514 has not yet been referenced specifically in any publications.

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Regarding ab5514, upon initial testing we have found our results to be unreliable in WB. During our testing we have sometimes detected the correct band of interest, but at other times we detect no band or...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (EPC1)
Loading amount 0.05 µg
Specification EPC1
Gel Running Conditions Reduced Denaturing (10% Acrylamide gel)
Blocking step Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 18 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (various incl: HEK293, HeLa, U2OS)
Loading amount 30 µg
Specification various incl: HEK293, HeLa, U2OS
Gel Running Conditions Reduced Denaturing (7.5% SDS-PAGE)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Oct 07 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"