Anti-EPC1 抗体 (ab112043)
Key features and details
- Rabbit polyclonal to EPC1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-EPC1 antibody -
製品の詳細
Rabbit polyclonal to EPC1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide, corresponding to a region between amino acids 325-375 of Human EPC1 (NP_079485.1).
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ポジティブ・コントロール
- HeLa whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
7 to 8 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab112043の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 93 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 93 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. -
配列類似性
Belongs to the enhancer of polycomb family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 450393 Chimpanzee
- Entrez Gene: 80314 Human
- Entrez Gene: 13831 Mouse
- Entrez Gene: 100362678 Rat
- Omim: 610999 Human
- SwissProt: Q9H2F5 Human
- SwissProt: Q8C9X6 Mouse
- Unigene: 167805 Human
see all -
別名
- DKFZp781P2312 antibody
- Enhancer of polycomb 1 antibody
- Enhancer of polycomb homolog 1 (Drosophila) antibody
see all
画像
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All lanes : Anti-EPC1 antibody (ab112043) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 93 kDa
Exposure time: 3 minutes -
Detection of EPC1 by Western Blot of Immunprecipitate.
ab112043 at 1µg/ml staining EPC1 in HeLa whole cell lysate immunoprecipitated using ab112043 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab112043 は 1 報の論文で使用されています。
- Wang Y et al. Epigenetic factor EPC1 is a master regulator of DNA damage response by interacting with E2F1 to silence death and activate metastasis-related gene signatures. Nucleic Acids Res 44:117-33 (2016). ChIP . PubMed: 26350215