製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to EPB4IL2
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Orangutan
  • 免疫原

    Synthetic peptide:

    RREVRSPTKAPH

    (Human) from the internal region of the protein sequence according to NP_001422.1.

  • ポジティブ・コントロール
    • Human Brain (Cerebellum) lysates

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    Peptide ELISA: Antibody detection limit dilution 1/32000.
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a double band of approximately 38-40 kDa (this band was successfully blocked by incubation with the immunising peptide) and the expected band of 113 kDa (predicted molecular weight: 113 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 組織特異性
      Widely expressed.
    • 配列類似性
      Contains 1 FERM domain.
    • 翻訳後修飾
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在
      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 4.1 G antibody
      • 4.1G antibody
      • AW555191 antibody
      • Band 4.1 like protein 2 antibody
      • Band 4.1-like protein 2 antibody
      • D10Ertd398e antibody
      • DKFZp781D1972 antibody
      • DKFZp781H1755 antibody
      • E41L2_HUMAN antibody
      • Epb41l2 antibody
      • Erythrocyte membrane protein band 4.1 like 2 antibody
      • Erythrocyte membrane protein band 4.1 like protein 2 antibody
      • Generally expressed protein 4.1 antibody
      • NBL2 antibody
      • OTTHUMP00000017197 antibody
      • OTTHUMP00000040262 antibody
      see all

    画像

    • All lanes : Anti-EPB4IL2 antibody (ab77263) at 0.1 µg/ml

      Lane 1 : Human cerebellum lysate in RIPA buffer
      Lane 2 : Human cerebellum lysate in RIPA buffer with immunising peptide

      Lysates/proteins at 35 µg/ml per lane.

      Developed using the ECL technique

      Predicted band size : 113 kDa
      Observed band size : 113 kDa
      Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    プロトコール

    参考文献

    ab77263 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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