製品の概要

  • 製品名Anti-EPB42 antibody
    EPB42 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to EPB42
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: KMPEKAEQYQ PLTASVSLQN SLDAPMEDCV ISILGRGLIH RERSYRFRSV WPENTMCAKF QFTPTHVGLQ RLTVEVDCNM FQNLTNYKSV TVVAPELSA, corresponding to amino acids 623-722 of Human EPB42 with a 26kDa enzyme tag (NP_000110).

  • ポジティブ・コントロール
    • EPB42 transfected 293T lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).

ターゲット情報

  • 機能Probably plays an important role in the regulation of erythrocyte shape and mechanical properties.
  • 関連疾患Defects in EPB42 are the cause of spherocytosis type 5 (SPH5) [MIM:612690]; also known as hereditary spherocytosis type 5 (HS5). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. Absence of band 4.2 associated with spur or target erythrocytes has also been reported.
  • 配列類似性Belongs to the transglutaminase superfamily. Transglutaminase family.
  • 翻訳後修飾Both cAMP-dependent kinase (CAPK) and another kinase present in the red-blood cells seem to be able to phosphorylate EPB42.
  • 細胞内局在Cell membrane. Cytoplasm > cytoskeleton. Cytoplasmic surface of erythrocyte membranes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EPB42 antibody
    • EPB42_HUMAN antibody
    • Erythrocyte membrane protein band 4.2 antibody
    • Erythrocyte protein 4.2 antibody
    • Erythrocyte surface protein band 4.2 antibody
    • MGC116735 antibody
    • MGC116737 antibody
    • P4.2 antibody
    • PA antibody
    • SPH5 antibody
    see all

Anti-EPB42 antibody 画像

  • Anti-EPB42 antibody (ab76910) at 1/500 dilution + Recombinant fragment of human EPB42 at 0.1 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP

    Predicted band size : 70 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-EPB42 antibody (ab76910) at 1/500 dilution

    Lane 1 : EPB42 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 70 kDa
    Observed band size : 70 kDa

Anti-EPB42 antibody (ab76910) 使用論文

ab76910 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"