製品の概要

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項（精製）
    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab78607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 81.9 kDa).
ELISA Use at an assay dependent concentration. Peptide ELISA antibody detection limit dilution 1:8000.

ターゲット情報

  • 機能
    May contribute to the correct positioning of tight junctions during the establishment of polarity in epithelial cells.
  • 配列類似性
    Contains 1 FERM domain.
  • ドメイン
    The FERM domain binds to CRB1.
  • 細胞内局在
    Cytoplasm. Cell junction > adherens junction. Associated with the plasma membrane. Colocalizes with CTNNB1, an adherens junction marker.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Band 4.1-like protein 5 antibody
      • BE37 antibody
      • E41L5_HUMAN antibody
      • Epb41l5 antibody
      • erythrocyte membrane protein band 4.1 like 5 antibody
      • KIAA1548 antibody
      see all

    画像

    • Western blot - Anti-EPB41L5 antibody (ab78607)
      Western blot - Anti-EPB41L5 antibody (ab78607)
      Anti-EPB41L5 antibody (ab78607) at 1 µg/ml + Human Brain (Hippocampus) Lysate in RIPA buffer at 35 µg

      Predicted band size: 81.9 kDa



      Primary incubation was 1 hour.

    プロトコール

    参考文献

    ab78607 has not yet been referenced specifically in any publications.

    ab78607 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    2 reviews or Q&A

    10
    Votes: Highest First

    myc-tagged human protein, 2 isoforms overexpressed in MDCK cells
    immunogen is present in construct
    can detect myc-tag, but not protein
    problem: no bands, only background
    cell lysate in 1% SDS, 30 ug loaded
    Ab: 1/125, 48 h 4 degree

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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    Non specific bands. Our customer tested WB with ab78607 and his samples were EPB41L5 gene transfected Hela cells. He expected bands on those transfected cells but, he also detected bands on non-transfected cells (control). whereas FLAG M2indicate...

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    Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody.

    The details your customer has kindly provided...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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