製品の概要

  • 製品名Anti-Epac2 antibody
    Epac2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Epac2
  • 特異性This antibody does not cross react with other Epac family proteins.
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Human
  • 免疫原

    Synthetic peptide:

    CPREQFDSLTPLPEQEGPT

    , corresponding to amino acids 743-762 of Epac2

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab21238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Predicted molecular weight: 112 kDa.
ELISA 1/10000.
ICC/IF Use at an assay dependent concentration. PubMed: 21799930

ターゲット情報

  • 機能Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.
  • 組織特異性Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.
  • 配列類似性Contains 2 cyclic nucleotide-binding domains.
    Contains 1 DEP domain.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 Ras-GEF domain.
  • ドメインThe N-terminal nucleotide phosphate binding region cAMP 1 has a much lower affinity for cAMP as compared to cAMP 2.
    The DEP domain is involved in membrane localization independent from regulation by cAMP.
  • 細胞内局在Cytoplasm. Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAMP GEFII antibody
    • cAMP regulated guanine nucleotide exchange factor II antibody
    • cAMP-GEFII antibody
    • cAMP-regulated guanine nucleotide exchange factor II antibody
    • CAMPGEFII antibody
    • CGEF 2 antibody
    • CGEF2 antibody
    • Epac 2 antibody
    • EPAC2 antibody
    • Exchange factor directly activated by cAMP 2 antibody
    • Exchange protein directly activated by cAMP 2 antibody
    • Nbla00496 antibody
    • Putative protein product of Nbla00496 antibody
    • Rap guanine nucleotide exchange factor (GEF) 4 antibody
    • Rap guanine nucleotide exchange factor 4 antibody
    • RAPGEF 4 antibody
    • Rapgef4 antibody
    • RPGF4_HUMAN antibody
    see all

Anti-Epac2 antibody 画像

  • ab21238 staining Epac2 in the bronchial surface epithelial 16HBE14o- cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were rinsed twice with phosphate-buffered saline (PBS) and then fixed with 4% paraformaldehyde for 30 minutes at 4°C, permeabilized with 0.5% Triton X-100 in PBS for 15 minutes and blocked with 10% fetal calf serum (FCS) in PBS for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 10% FCS) overnight at 4°C. An Alex Fluor®568-conjugated Goat anti-rabbit IgG was used as the secondary antibody. The corresponding image taken in brightfield is shown on the right.

  • Anti-Epac2 antibody (ab21238) at 1/1000 dilution + Whole cell lysates prepared from rabbit brain (positive control) and kidney, pancreas and pancretic acini (negative controls) at 20 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG

    Performed under reducing conditions.

    Predicted band size : 112 kDa


    Exposure time : 3 minutes

    This image is courtesy of an Abreview submitted by Dr maria sabbatini

    See Abreview

Anti-Epac2 antibody (ab21238) 使用論文

This product has been referenced in:
  • Guibinga GH  et al. Deficiency of the purine metabolic gene HPRT dysregulates microRNA-17 family cluster and guanine-based cellular functions: a role for EPAC in Lesch-Nyhan syndrome. Hum Mol Genet 22:4502-15 (2013). Read more (PubMed: 23804752) »
  • Lau WK  et al. Differential Inhibitory Effects of CysLT(1) Receptor Antagonists on P2Y(6) Receptor-Mediated Signaling and Ion Transport in Human Bronchial Epithelia. PLoS One 6:e22363 (2011). ICC/IF ; Human . Read more (PubMed: 21799837) »

See all 4 Publications for this product

Product Wall

Thank you for providing that information.

Both the Abreview and the reference that are on the webpage for ab21238 use 5% BSA as a blocking agent and I am wondering if using that rather than 5% milk will help get a signal.

I would ad...

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Thank you for contacting Abcam.

I am sorry that you are having problems with ab21238 in western blot. To be able to help you more with this problem, I was wondering if you could give me a little more information:

1- How much sample ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rabbit Cell lysate - whole cell (pancreatic acini)
Loading amount 20 µg
Specification pancreatic acini
Gel Running Conditions Reduced Denaturing (7.5)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. maria sabbatini

Verified customer

投稿 Sep 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"