Rabbit polyclonal to Epac2
At least two isoforms of Epac2 are known to exist; this antibody will detect only the larger isoform.
Mouse, Rat, Human
Synthetic peptide corresponding to 15 amino acids near the N-terminus of Human Epac2 (NP_008954).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2.5 µg/ml.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 116 kDa.Can be blocked with
Epac2 peptide (ab213603).
Use a concentration of 20 µg/ml.
Use at an assay dependent concentration.
Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.
Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.
Contains 2 cyclic nucleotide-binding domains.
Contains 1 DEP domain. Contains 1 N-terminal Ras-GEF domain. Contains 1 Ras-GEF domain.
The N-terminal nucleotide phosphate binding region cAMP 1 has a much lower affinity for cAMP as compared to cAMP 2.
The DEP domain is involved in membrane localization independent from regulation by cAMP.
Information by UniProt
CAMP GEFII antibody
cAMP regulated guanine nucleotide exchange factor II antibody
Immunocytochemistry/ Immunofluorescence - Anti-Epac2 antibody (ab124189)
Immunofluorescence of Epac2 in mouse liver tissue with ab124189 at 20 ug/mL.
Western blot - Anti-Epac2 antibody (ab124189)
All lanes : Anti-Epac2 antibody (ab124189) at 1 µg/ml Lane 1 : Rat liver tissue lysate Lane 2 : Rat liver tissue lysate with blocking peptide Lysates/proteins at 15 µg per lane. Predicted band size : 116 kDa
Immunohistochemistry - Anti-Epac2 antibody (ab124189)
Immunohistochemistry of EPAC2 in mouse liver tissue with ab124189 at 2.5 ug/mL.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"