製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to Eotaxin 2
  • アプリケーション
    適用あり: ELISA, Sandwich ELISA, WB, Neutralisingmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Highly pure (>98%) recombinant mouse Eotaxin 2

製品の特性

  • 製品の状態
    Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab84016 is sterile filtered and was purified by affinity chromatography employing immobilized mouse Eotaxin 2 matrix.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab84016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant mouse Eotaxin 2.
Sandwich ELISA Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Goat polyclonal to Eotaxin 2 (Biotin) (ab84015). Allows the detection of at least 0.2 - 0.4 ng/well of recombinant mouse Eotaxin 2. Can be used as Capture antibody when paired with ab84015.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 13 kDa. The detection limit for recombinant mouse Eotaxin 2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralising Use a concentration of 7 - 8 µg/ml. To yield one-half maximal inhibition [ND50] of the biological activity of mouse Eotaxin 2 (100 ng/ml).

ターゲット情報

  • 機能
    Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3.
  • 組織特異性
    Activated monocytes and activated T lymphocytes.
  • 配列類似性
    Belongs to the intercrine beta (chemokine CC) family.
  • 翻訳後修飾
    N-glycosylated.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C-C motif chemokine 24 antibody
    • CCL24 antibody
    • CCL24_HUMAN antibody
    • Chemokine CC Motif Ligand 24 antibody
    • CK beta 6 antibody
    • CK-beta-6 antibody
    • Ckb6 antibody
    • Eosinophil chemotactic protein 2 antibody
    • Eotaxin-2 antibody
    • MPIF-2 antibody
    • MPIF2 antibody
    • Myeloid progenitor inhibitory factor 2 antibody
    • SCYA24 antibody
    • Small inducible cytokine subfamily A (Cys-Cys) member 24 antibody
    • Small-inducible cytokine A24 antibody
    see all

プロトコール

参考文献

ab84016 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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