Anti-ENY2 抗体 (ab121836)
Key features and details
- Rabbit polyclonal to ENY2
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ENY2 antibody -
製品の詳細
Rabbit polyclonal to ENY2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
antigen sequence: AINQKLMETG ERERLKELLR AKLIECGWKD QLKAHCKEVI KEKGLEHVTV DDLVAEITPK GRALVPDSVK KELLQRIRT, corresponding to amino acids 15-93 of Human ENY2.
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ポジティブ・コントロール
- Human rectum tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121836の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Component of the transcription regulatory histone acetylation (HAT) complex SAGA, a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates to a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. May also participate in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. -
配列類似性
Belongs to the ENY2 family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 56943 Human
- SwissProt: Q9NPA8 Human
- Unigene: 492555 Human
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別名
- 1810057B09Rik antibody
- 6720481I12 antibody
- DC6 antibody
see all
画像
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Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and mitochondria. Recommended concentration of ab121836 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab121836 at 1/40 dilution staining ENY2 in Paraffin-embedded Human Rectum tissue by Immunohistochemistry. Note: strong positivity in glandular cells.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab121836 は論文での使用が確認できていません。