The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 48 kDa).Can be blocked with ENTPD5 peptide (ab190069).
1/10 - 1/100.
1/100 - 1/250. Antigen retrieval is recommended.
Is unsuitable for Flow Cyt or ICC.
Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. Also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.
Expressed in adult liver, kidney, prostate, testis and colon. Much weaker expression in other tissues.
Walsh J et al. Identification and quantification of the basal and inducible Nrf2-dependent proteomes in mouse liver: biochemical, pharmacological and toxicological implications. J Proteomics108:171-87 (2014).
Read more (PubMed: 24859727) »
Curry NL et al. Pten-null tumors cohabiting the same lung display differential AKT activation and sensitivity to dietary restriction. Cancer Discov3:908-21 (2013).
Read more (PubMed: 23719831) »