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RabMAb

Anti-ENTPD5 抗体 [EPR3784] (ab108603)

製品の概要

  • 製品名
    Anti-ENTPD5 antibody [EPR3784]
    ENTPD5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3784] to ENTPD5
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • ポジティブ・コントロール
    • Fetal liver cell lysate; Human kidney tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 48 kDa).Can be blocked with ENTPD5 peptide (ab190069).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
  • 追加情報
    Is unsuitable for Flow Cyt or ICC.
  • ターゲット情報

    • 機能
      Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. Also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.
    • 組織特異性
      Expressed in adult liver, kidney, prostate, testis and colon. Much weaker expression in other tissues.
    • パスウェイ
      Protein modification; protein glycosylation.
    • 配列類似性
      Belongs to the GDA1/CD39 NTPase family.
    • 翻訳後修飾
      N-glycosylated; high-mannose type.
    • 細胞内局在
      Endoplasmic reticulum lumen.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD39 like 4 antibody
      • AI196558 antibody
      • AI987697 antibody
      • CD39 antigen like 4 antibody
      • CD39 antigen-like 4 antibody
      • CD39 like 4 antibody
      • CD39L4 antibody
      • Ectonucleoside triphosphate diphosphohydrolase 5 antibody
      • ENTP5_HUMAN antibody
      • Entpd5 antibody
      • ER UDPase antibody
      • ER-UDPase antibody
      • GDPase ENTPD5 antibody
      • Guanosine-diphosphatase ENTPD5 antibody
      • MGC163357 antibody
      • MGC163359 antibody
      • mNTPase antibody
      • NTPDase 5 antibody
      • Nucleoside diphosphatase antibody
      • PCPH antibody
      • Pcph proto oncogene protein antibody
      • Proto oncogene CPH antibody
      • UDPase ENTPD5 antibody
      • Uridine-diphosphatase ENTPD5 antibody
      see all

    画像

    • Anti-ENTPD5 antibody [EPR3784] (ab108603) at 1/1000 dilution + fetal liver cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 48 kDa
      Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    • ab108603, at 1/100 dilution, staining ENTPD5 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    参考文献

    This product has been referenced in:
    • Walsh J  et al. Identification and quantification of the basal and inducible Nrf2-dependent proteomes in mouse liver: biochemical, pharmacological and toxicological implications. J Proteomics 108:171-87 (2014). WB ; Mouse . Read more (PubMed: 24859727) »
    • Curry NL  et al. Pten-null tumors cohabiting the same lung display differential AKT activation and sensitivity to dietary restriction. Cancer Discov 3:908-21 (2013). WB, IHC-P ; Mouse . Read more (PubMed: 23719831) »

    See all 2 Publications for this product

    レビューと Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Mouse Cell (Spermatozoa)
    Specification
    Spermatozoa
    Permeabilization
    Yes - Triton X 0.25%
    Fixative
    Paraformaldehyde
    Username

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    投稿 May 30 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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