Anti-ENPP3 抗体 [NP4D6] (Phycoerythrin) (ab90751)

製品の概要

  • 製品名Anti-ENPP3 antibody [NP4D6] (Phycoerythrin)
    ENPP3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [NP4D6] to ENPP3 (Phycoerythrin)
  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm
  • アプリケーション適用あり: Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    HEK-293 cells transfected with Human ENPP3

  • 特記事項

    The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab90751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
ICC/IF
  • 追加情報Flow Cyt: Use 20µl for 106 cells.
    ICC/IF: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD.
    • 組織特異性Expressed in bile ducts, prostate, uterus and colon. Exclusively expressed on basophils, mast cells and their progenitors.
    • 配列類似性Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
      Contains 2 SMB (somatomedin-B) domains.
    • 翻訳後修飾N-glycosylation is necessary for correct trafficking to the apical surface, but is not the apical targeting signal.
      It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    • 細胞内局在Membrane. Secreted. Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Alkaline phosphodiesterase I antibody
      • ARHR2 antibody
      • B10 antibody
      • CD203c antibody
      • CD203c antigen antibody
      • dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) antibody
      • dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) antibody
      • E NPP 3 antibody
      • E-NPP 3 antibody
      • Ectonucleotide pyrophosphatase/phosphodiesterase 3 antibody
      • Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 antibody
      • ENPP3 antibody
      • ENPP3_HUMAN antibody
      • gp130RB13 6 antibody
      • gp130RB136 antibody
      • M6S1 antibody
      • NPP1 antibody
      • NPP3 antibody
      • NPPase antibody
      • NPPS antibody
      • Nucleotide pyrophosphatase antibody
      • PC-1 antibody
      • PCA1 antibody
      • PD Ibeta antibody
      • PD-Ibeta antibody
      • PDNP1 antibody
      • PDNP3 antibody
      • Phosphodiesterase I beta antibody
      • Phosphodiesterase I/nucleotide pyrophosphatase 3 antibody
      • phosphodiesterase i/nucleotide pyrophosphatase beta antibody
      see all

    Anti-ENPP3 antibody [NP4D6] (Phycoerythrin) 画像

    • ab90751 staining ENPP3 on the surface of human basophils isolated from allergen-stimulated whole blood by flow cytometery.

    • Surface staining of activated human basophil by FITC-conjugated anti-CD63 (clone MEM-259, ab18235, green) and PE-conjugated anti-ENPP3 (clone NP4D6, ab90751, red); merged signal yellow. DNA visualized by DAPI (blue).

    Anti-ENPP3 antibody [NP4D6] (Phycoerythrin) (ab90751) 使用論文

    ab90751 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Fixation no fixation
    Permeabilization No
    Sample Human Cell (human basophile granulocytes)
    Specification human basophile granulocytes
    Gating Strategy leucocyte gate
    Preparation Cell harvesting/tissue preparation method: peripheral blood cells, separated by Ficoll density separation, followed by magnetic isolation
    Sample buffer: PBS with human IgG (Fc-block)
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    投稿 Aug 15 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Sample Human Cell (peripheral blood cells)
    Specification peripheral blood cells
    Permeabilization No
    Fixative Methanol
    Username

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    Verified customer

    投稿 Aug 15 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (PBMC)
    Specification PBMC
    Preparation Cell harvesting/tissue preparation method: Ficoll-hypaque gradrient
    Sample buffer: PBS
    Permeabilization No
    Gating Strategy lymphocytes
    Username

    Abcam user community

    Verified customer

    投稿 Feb 19 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"