製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to ENOX2
  • 特異性
    ab77482 is expected to recognise both reported isoforms (NP_006366.2; NP_872114.1).
  • アプリケーション
    適用あり: ELISA, IHC-Pmore details
  • 種交差性

    交差が予測される動物種: Cow, Dog, Human, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    C-EKLKDDKLQVEK

    , from an internal region of Human ENOX2 (NP_006366.2; NP_872114.1).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab77482 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
IHC-P
  • 追加情報
    Peptide ELISA: Antibody detection limit dilution 1/64000.
    WB: Preliminary experiments gave bands at approx 60kDa and 37kDa in Human Heart lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 66.6kDa and 70.1kDa according to NP_006366.2 and NP_872114.1. Both detected bands were successfully blocked by incubation with the immunising peptide (and BLAST results with the immunising peptide sequence did not identify any other proteins to explain the additional bands).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock.
    • 組織特異性
      Found in the sera of cancer patients with a wide variety of cancers including breast, prostate, lung and ovarian cancers, leukemias, and lymphomas. Not found in the serum of healthy volunteers or patients with disorders other than cancer. Probably shed into serum by cancer cells. Found on the cell borders of renal, kidney and ovarian carcinomas but not on the borders of surrounding non-cancerous stromal cells.
    • 配列類似性
      Belongs to the ENOX family.
      Contains 1 RRM (RNA recognition motif) domain.
    • 翻訳後修飾
      Glycosylated.
    • 細胞内局在
      Cell membrane. Secreted > extracellular space. Extracellular and plasma membrane-associated.
    • Information by UniProt
    • 参照データベース
    • 別名
      • APK1 antibody
      • APK1 antigen antibody
      • COVA1 antibody
      • Cytosolic ovarian carcinoma antigen 1 antibody
      • Ecto NOX disulfide thiol exchanger 2 antibody
      • ENOX 2 antibody
      • ENOX2 antibody
      • ENOX2_HUMAN antibody
      • OTTHUMP00000024021 antibody
      • OTTHUMP00000024022 antibody
      • Protein disulfide-thiol oxidoreductase antibody
      • tNOX antibody
      • Tumor associated hydroquinone oxidase antibody
      • Tumor-associated hydroquinone oxidase antibody
      see all

    画像

    • Immunohistochemistry (paraffin-embedded sections) analysis of human kidney labeling ENOX2 with ab77482 at 4µg/ml. Microwaved antigen retrieval was performed using Tris/EDTA buffer pH9. HRP-staining.

    プロトコール

    参考文献

    ab77482 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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