製品の概要

  • 製品名Anti-eNOS (phospho S632) antibody [M232]
    eNOS 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [M232] to eNOS (phospho S632)
  • 特異性ab76199 is not expected to react with other NOS family members due to low homology.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic phosphopeptide corresponding to amino acids surrounding Ser632 in mouse eNOS, coupled to carrier protein. This sequence is conserved in human (Ser633) and rat (Ser632) eNOS.

  • ポジティブ・コントロール
    • Human umbilical vein endothelial cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 140, 120 kDa (predicted molecular weight: 133 kDa).
ELISA 1/1000.

ターゲット情報

  • 機能Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
    Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
  • 組織特異性Platelets, placenta, liver and kidney.
  • 関連疾患Variation in NOS3 seem to be associated with susceptibility to coronary spasm.
  • 配列類似性Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • 翻訳後修飾Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
  • 細胞内局在Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cNOS antibody
    • Constitutive NOS antibody
    • EC NOS antibody
    • EC-NOS antibody
    • ecNOS antibody
    • Endothelial nitric oxidase synthase antibody
    • Endothelial nitric oxide synthase antibody
    • Endothelial nitric oxide synthase 3 antibody
    • Endothelial NOS antibody
    • eNOS antibody
    • Nitric oxide synthase 3 (endothelial cell) antibody
    • Nitric oxide synthase 3 antibody
    • Nitric oxide synthase 3 endothelial cell antibody
    • Nitric oxide synthase endothelial antibody
    • Nitric oxide synthase, endothelial antibody
    • NOS 3 antibody
    • NOS III antibody
    • NOS type III antibody
    • NOS3 antibody
    • NOS3_HUMAN antibody
    • NOSIII antibody
    see all

Anti-eNOS (phospho S632) antibody [M232] 画像

  • All lanes : Anti-eNOS (phospho S632) antibody [M232] (ab76199) at 1/500 dilution

    Lane 1 : human umbilical vein endothelial cells, untreated
    Lane 2 : human umbilical vein endothelial cells, treated with lambda phosphatase


    Predicted band size : 133 kDa
    Observed band size : 120,140 kDa (why is the actual band size different from the predicted?)

Anti-eNOS (phospho S632) antibody [M232] (ab76199) 使用論文

This product has been referenced in:
  • Tobin GA  et al. The Role of eNOS Phosphorylation in Causing Drug-induced Vascular Injury. Toxicol Pathol 42:709-724 (2014). Read more (PubMed: 24705881) »
  • Niu X  et al. ß3-adrenoreceptor stimulation protects against myocardial infarction injury via eNOS and nNOS activation. PLoS One 9:e98713 (2014). WB ; Mouse . Read more (PubMed: 24911015) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"