製品の概要

  • 製品名Anti-eNOS (phospho S1177) antibody
    eNOS 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to eNOS (phospho S1177)
  • 特異性This antibody detects endogenous levels of eNOS only when phosphorylated at serine 1176
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human eNOS (phospho S1177).

  • ポジティブ・コントロール
    • HeLa cell extract

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).
ELISA 1/20000.

ターゲット情報

  • 機能Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
    Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
  • 組織特異性Platelets, placenta, liver and kidney.
  • 関連疾患Variation in NOS3 seem to be associated with susceptibility to coronary spasm.
  • 配列類似性Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • 翻訳後修飾Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
  • 細胞内局在Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cNOS antibody
    • Constitutive NOS antibody
    • EC NOS antibody
    • EC-NOS antibody
    • ecNOS antibody
    • Endothelial nitric oxidase synthase antibody
    • Endothelial nitric oxide synthase antibody
    • Endothelial nitric oxide synthase 3 antibody
    • Endothelial NOS antibody
    • eNOS antibody
    • Nitric oxide synthase 3 (endothelial cell) antibody
    • Nitric oxide synthase 3 antibody
    • Nitric oxide synthase 3 endothelial cell antibody
    • Nitric oxide synthase endothelial antibody
    • Nitric oxide synthase, endothelial antibody
    • NOS 3 antibody
    • NOS III antibody
    • NOS type III antibody
    • NOS3 antibody
    • NOS3_HUMAN antibody
    • NOSIII antibody
    see all

Anti-eNOS (phospho S1177) antibody 画像

  • All lanes : Anti-eNOS (phospho S1177) antibody (ab51038) at 1/500 dilution

    Lane 1 : HeLa cell extract treated with Insulin (0.01 U/ml for 15min), with the immunising peptide
    Lane 2 : HeLa cell extract treated with Insulin (0.01 U/ml for 15min), with the immunising peptide


    Predicted band size : 133 kDa
    Observed band size : 133 kDa

Anti-eNOS (phospho S1177) antibody (ab51038) 使用論文

This product has been referenced in:
  • Cheang WS  et al. Endothelial nitric oxide synthase enhancer reduces oxidative stress and restores endothelial function in db/db mice. Cardiovasc Res 92:267-75 (2011). Read more (PubMed: 21875904) »

See 1 Publication for this product

Product Wall

Thank you for your patience. I managed to locate a publication that used endothelia from aorta of rats for an eNOS (phospho S1176) experiment. Therefore, this can also be used as a positive control for ab51038. Please see the Materials and Meth...

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Thank you for your enquiry. I can see from the SwissProt database entry for this protein (http://us.expasy.org/cgi-bin/niceprot.pl?P29474) that they list amino acid 1177 as being a phosphoserine. If you look at the sequence, however, they are incl...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"