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Our Abpromise guarantee covers the use of ab15280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Glut||1/1. PubMed: 19926873|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Ab15280 (used neat) staining human B-lineage Lymphoma tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval, the antibody was incubated with the tissue for 1 hour. Bound antibody was detected using a biotinylated horse anti-mouse and rabbit IgG antibody.
This image is courtesy of an Abreview submitted by Francisca Wu on 10 March 2006.