製品の概要

  • 製品名Anti-eNOS antibody [NOS-E1]
    eNOS 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [NOS-E1] to eNOS
  • 特異性ab50010 reacts specifically with endothelial-type nitric oxide synthase (eNOS). It does not react with NOS derived from macrophages (mNOS) and brain (bNOS). Please note that this is an IgA antibody and therefore requires an anti-mouse IgA secondary antibody for detection.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
    交差が予測される動物種: Sheep, Rabbit, Guinea pig, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to nitric oxide synthase (NOS) from bovine endothelial origin (eNOS, amino acids 1185-1205 with an N-terminally added lysine) conjugated to KLH.

  • ポジティブ・コントロール
    • Whole cell extract of cultured bovine lung endothelial cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Predicted molecular weight: 133 kDa.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
    Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
  • 組織特異性Platelets, placenta, liver and kidney.
  • 関連疾患Variation in NOS3 seem to be associated with susceptibility to coronary spasm.
  • 配列類似性Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • 翻訳後修飾Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
  • 細胞内局在Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cNOS antibody
    • Constitutive NOS antibody
    • EC NOS antibody
    • EC-NOS antibody
    • ecNOS antibody
    • Endothelial nitric oxidase synthase antibody
    • Endothelial nitric oxide synthase antibody
    • Endothelial nitric oxide synthase 3 antibody
    • Endothelial NOS antibody
    • eNOS antibody
    • Nitric oxide synthase 3 (endothelial cell) antibody
    • Nitric oxide synthase 3 antibody
    • Nitric oxide synthase 3 endothelial cell antibody
    • Nitric oxide synthase endothelial antibody
    • Nitric oxide synthase, endothelial antibody
    • NOS 3 antibody
    • NOS III antibody
    • NOS type III antibody
    • NOS3 antibody
    • NOS3_HUMAN antibody
    • NOSIII antibody
    see all

Anti-eNOS antibody [NOS-E1] 画像

  • All lanes : Anti-eNOS antibody [NOS-E1] (ab50010) at 1/500 dilution

    Lane 1 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
    Lane 2 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgA secondary at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 133 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

Anti-eNOS antibody [NOS-E1] (ab50010) 使用論文

This product has been referenced in:
  • Marrachelli VG  et al. Role of NO-synthases and cyclooxygenases in the hyperreactivity of male rabbit carotid artery to testosterone under experimental diabetes. Pharmacol Res 61:62-70 (2010). WB ; Rabbit . Read more (PubMed: 19573602) »

See 1 Publication for this product

Product Wall

Thank you for your call today and for letting us know about the trouble with ab 50010. As we discussed, I am sending a free of charge replacement vial of ab76198 on the order ***, which should arrive tomorrow. Please keep me updated with any new result...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"