製品の概要

  • 製品名Anti-eNOS antibody
    eNOS 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to eNOS
  • 特異性This antibody is specific for nitric oxide synthase (NOS), derived from endothelial tissue, but does not recognize NOS derived from brain (bNOS) and macrophages (iNOS).
  • アプリケーション適用あり: WB, IHC-Fr, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Cow
    交差が予測される動物種: Rat, Guinea pig, Human
  • 免疫原

    Synthetic peptide:

    SGPYNSSPRPEQHK

    conjugated to KLH, corresponding to internal sequence amino acids 596-609 of Cow eNOS

  • ポジティブ・コントロール
    • Whole extract of bovine lung endothelial cells or mouse heart tissue sections.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab50260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000. Predicted molecular weight: 133 kDa.
IHC-Fr 1/100. Fix with acetone.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20702812

ターゲット情報

  • 機能Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
    Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
  • 組織特異性Platelets, placenta, liver and kidney.
  • 関連疾患Variation in NOS3 seem to be associated with susceptibility to coronary spasm.
  • 配列類似性Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • 翻訳後修飾Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
  • 細胞内局在Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cNOS antibody
    • Constitutive NOS antibody
    • EC NOS antibody
    • EC-NOS antibody
    • ecNOS antibody
    • Endothelial nitric oxidase synthase antibody
    • Endothelial nitric oxide synthase antibody
    • Endothelial nitric oxide synthase 3 antibody
    • Endothelial NOS antibody
    • eNOS antibody
    • Nitric oxide synthase 3 (endothelial cell) antibody
    • Nitric oxide synthase 3 antibody
    • Nitric oxide synthase 3 endothelial cell antibody
    • Nitric oxide synthase endothelial antibody
    • Nitric oxide synthase, endothelial antibody
    • NOS 3 antibody
    • NOS III antibody
    • NOS type III antibody
    • NOS3 antibody
    • NOS3_HUMAN antibody
    • NOSIII antibody
    see all

Anti-eNOS antibody (ab50260) 使用論文

This product has been referenced in:
  • Feng J  et al. Endothelium-dependent coronary vasodilatation requires NADPH oxidase-derived reactive oxygen species. Arterioscler Thromb Vasc Biol 30:1703-10 (2010). IHC-FoFr ; Mouse . Read more (PubMed: 20702812) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Mesentery sections)
Permeabilization Yes - Triton X-100
Specification Mesentery sections
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Fixative Acetone
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投稿 Nov 24 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"