製品の概要

  • 製品名
    ENO1 Human Activity Assay Kit
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Cell extract 9 9%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Cell extract 4 11%
  • サンプルの種類
    Cell culture extracts, Tissue Extracts
  • アッセイタイプ
    Enzyme activity
  • 検出感度
    125 ng/ml
  • 検出範囲
    125 ng/ml - 2000 ng/ml
  • 添加回収試験

    90 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 63 % - %
    Serum 140 % - %

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 製品の概要

    ab117994 is used to determine enolase 1 (ENO1) activity in a sample. The native enzyme is immunocaptured within the wells of the microplate; this removes all other enzymes. The enolase activity is determined via a coupled reaction to the consumption of NADH in an assay buffer.

  • アプリケーション
    適用あり: Functional Studiesmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab117994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

画像

  • Functional Studies - ENO1 Human Activity Assay Kit (ab117994)
    Functional Studies - ENO1 Human Activity Assay Kit (ab117994)
    Analysis of recombinant ENO1 (ab89248) and the neuron-specific recombinant ENO2 (ab78797), each at 2000 ng/mL. The relative ENO activity was determined from HepG2 standard curve as described in the protocol. The data show that the activity of the neuron-specific ENO2 can’t be detected.
  • Functional Studies - ENO1 Human Activity Assay Kit (ab117994)
    Functional Studies - ENO1 Human Activity Assay Kit (ab117994)
    Example of dilution series prepared from HepG2 cells extract.
  • Immunocytochemistry/ Immunofluorescence - ENO1 Human Activity Assay Kit (ab117994)
    Immunocytochemistry/ Immunofluorescence - ENO1 Human Activity Assay Kit (ab117994)
    Immunofluorescent detection of ENO1 in cytosol in HeLa cells using ab112994.
  • Functional Studies - ENO1 Human Activity Assay Kit (ab117994)
    Functional Studies - ENO1 Human Activity Assay Kit (ab117994)
    Scheme of reactions used in this kit. Abbreviations are as follows: enolase (ENO), 2-phospho-D-glycerate (2PG), phosphoenolpyruvate (PEP), pyruvate kinase (PK), lactate dehydrogenase (LDH).

プロトコール

参考文献

This product has been referenced in:
  • Marchesini MI  et al. A T4SS Effector Targets Host Cell Alpha-Enolase Contributing to Brucella abortus Intracellular Lifestyle. Front Cell Infect Microbiol 6:153 (2016). Read more (PubMed: 27900285) »
  • Bottermann K  et al. Systematic Analysis Reveals Elongation Factor 2 and a-Enolase as Novel Interaction Partners of AKT2. PLoS One 8:e66045 (2013). Read more (PubMed: 23823123) »

See all 2 Publications for this product

ab117994 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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2 reviews or Q&A

10
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Can I have a more detailed protocol regarding how to do the data analysis for a kinetic assay? Also, please provide details as to the proper use of the LDH control in the assay.

Regarding LDH control:
Please note that the LDH is simply added to the rehydrated 'Reagent Mix', along with PK, to produce the 1x Acitvity Solution.

Regarding data analysis:
If data collected in a kinetic mode, they are presented as...

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Welche Platte wird in ab117994 verwendet?

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Bitte entschuldigen Sie die verspätete Antwort. MitoSciences sitzt an der Westküste in den USA und durch die Zeitverschiebung habe ich erst jetzt eine Antwort erhalten.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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