Anti-ENO1 抗体 (ab97586)
Key features and details
- Rabbit polyclonal to ENO1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-ENO1 antibody
ENO1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ENO1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Guinea pig, Zebrafish -
免疫原
Recombinant fragment containing a sequence corresponding to a region within amino acids 199-412 of Human ENO1 (NP_001419).
-
ポジティブ・コントロール
- A431 and H1299 whole cell lysates; HeLa cells; 293T, HepG2, Molt-4 and Raji cell lines.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97586の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
|
|
ICC/IF |
1/100 - 1/200.
|
特記事項 |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
-
機能
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. -
組織特異性
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
パスウェイ
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
配列類似性
Belongs to the enolase family. -
発生段階
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
翻訳後修飾
ISGylated. -
細胞内局在
Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line. - Information by UniProt
-
参照データベース
- Entrez Gene: 2023 Human
- Entrez Gene: 24333 Rat
- Omim: 172430 Human
- SwissProt: P06733 Human
- SwissProt: P04764 Rat
- Unigene: 517145 Human
- Unigene: 4236 Rat
-
別名
- 2 phospho D glycerate hydro lyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Alpha enolase antibody
see all
画像
-
All lanes : Anti-ENO1 antibody (ab97586) at 1/3000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa
10% SDS PAGE -
ab97586, at 1/200 dilution, staining ENO1 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is costained with Hoechst 33342.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab97586 は論文での使用が確認できていません。