Anti-ENO1 抗体 [2G2AG11BF8] (ab112994)


  • 製品名
    Anti-ENO1 antibody [2G2AG11BF8]
    ENO1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2G2AG11BF8] to ENO1
  • アプリケーション
    適用あり: IP, In-Cell ELISA, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
    非交差種: Non human primates
  • 免疫原

    Tissue, cells or virus corresponding to ENO1 aa 1-434. The immunogen for this antibody was the cytosolic fraction of HeLa cells. The exact epitope sequence that this antibody recognizes is unknown.

  • ポジティブ・コントロール
    • HeLa and HL60 cells; HeLa, Human Liver and Heart homogenates.
  • 特記事項

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.



Our Abpromise guarantee covers the use of ab112994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
In-Cell ELISA Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.


  • 機能
    Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
    MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
  • 組織特異性
    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • パスウェイ
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • 配列類似性
    Belongs to the enolase family.
  • 発生段階
    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • 翻訳後修飾
  • 細胞内局在
    Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2 phospho D glycerate hydro lyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Alpha enolase antibody
    • Alpha enolase like 1 antibody
    • Alpha-enolase antibody
    • C myc promoter binding protein antibody
    • C-myc promoter-binding protein antibody
    • EC antibody
    • eno1 antibody
    • ENO1L1 antibody
    • ENOA_HUMAN antibody
    • Enolase 1 (alpha) antibody
    • Enolase 1 (alpha) like 1 antibody
    • Enolase 1 antibody
    • Enolase alpha antibody
    • MBP 1 antibody
    • MBP-1 antibody
    • MBP1 antibody
    • MBPB1 antibody
    • MPB 1 antibody
    • MPB-1 antibody
    • MPB1 antibody
    • MYC promoter binding protein 1 antibody
    • NNE antibody
    • Non neural enolase antibody
    • Non-neural enolase antibody
    • Phosphopyruvate hydratase antibody
    • Plasminogen binding protein antibody
    • Plasminogen-binding protein antibody
    • PPH antibody
    • Tau crystallin antibody
    see all

Anti-ENO1 antibody [2G2AG11BF8] 画像

  • Immunprecipitation of ENO1 by ab112994.
    Staining of ENO1 (47 kDa) from HeLa cell lysate (1), Human liver (2) and Heart (3) homogenates. The identity of the protein was confirmed by mass spectrometry. This gel was stained with colloidal Coomassie blue G.
  • ab112994 at 1ug/ml staining ENO1 in MeOH-fixed HL60 cells by Flow Cytometry(blue). Isotype control antibody (red).
  • ab112994 at 5ug/ml staining ENO1 in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green). The target locates mainly to the cytoplasm.

Anti-ENO1 antibody [2G2AG11BF8] (ab112994) 使用論文

ab112994 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your recent telephone enquiry. I am sorry it has taken some time to obtain this information, I appreciate your patience.

I can confirm that ab112994 was produced in vitro using hybridomas grown in serum-free medium, and then pu...

Read More