Anti-Enkephalin/ENK 抗体 (ab77273)
Key features and details
- Goat polyclonal to Enkephalin/ENK
- Suitable for: WB, IHC-FoFr
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Enkephalin/ENK antibody
Enkephalin/ENK 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Enkephalin/ENK -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-FoFrmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human Adrenal Gland lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77273の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa).
1 hour primary incubation is recommended for this product. |
|
IHC-FoFr |
Use at an assay dependent concentration.
|
特記事項 |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa). 1 hour primary incubation is recommended for this product. |
IHC-FoFr
Use at an assay dependent concentration. |
ターゲット情報
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機能
Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. -
配列類似性
Belongs to the opioid neuropeptide precursor family. -
翻訳後修飾
The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 5179 Human
- Omim: 131330 Human
- SwissProt: P01210 Human
- Unigene: 339831 Human
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別名
- ENK antibody
- Enkephalin A antibody
- Enkephalin antibody
see all
画像
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Anti-Enkephalin/ENK antibody (ab77273) at 0.3 µg/ml + Human Adrenal Gland lysate in RIPA buffer at 35 µg
Predicted band size: 31 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab77273 は 2 報の論文で使用されています。
- Shi MM et al. Hippocampal µ-opioid receptors on GABAergic neurons mediate stress-induced impairment of memory retrieval. Mol Psychiatry 25:977-992 (2020). PubMed: 31142818
- Tang D et al. High expression of proenkephalin is associated with favorable outcomes in patients with gastrointestinal stromal tumors. Cancer Manag Res 11:6681-6690 (2019). PubMed: 31410059