製品の概要

  • 製品名
    Anti-Engrailed and Invected antibody [4D9]
  • 製品の詳細
    Mouse monoclonal [4D9] to Engrailed and Invected
  • 特異性
    This antibody recognizes both the engrailed and invected gene products of Drosophila. The antibody is useful for examining the patterning of segmentation from the cellular blastoderm stage onward. Staining is also seen in a large number of CNS neurons including most of the median neuroblast progeny as well as a small number of PNS neurons.
  • アプリケーション
    適用あり: IHC-FoFrmore details
  • 種交差性
    交差種: Chicken, Xenopus laevis, Drosophila melanogaster, Zebrafish, Crayfish
    非交差種: Mouse
  • 免疫原

    C-terminal two-thirds of the invected protein were generated in E. coli with the T7 polymerase expression system.

  • エピトープ
    Epitope has been localized to residues 38-58 of the homeodomain.
  • 特記事項

    This antibody clone is manufactured by Abcam.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    モノクローナル
  • クローン名
    4D9
  • ミエローマ
    NS1
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab12454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/200. Fix with 4% paraformaldehyde in PBS for 1 hour.

ターゲット情報

  • 関連性
    In Drosophila, the Engrailed gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Expression studies have suggested that Engrailed may also function early in organizing the preblastoderm and later in neurogenesis. Engrailed encodes a homeodomain-containing protein that can function as a transcription factor. Invected is homologous to Engrailed and the gene lies adjacent to that for Engrailed. The function of Invected is unclear.
  • 参照データベース
    • 別名
      • En antibody
      • Eng antibody
      • Engrailed antibody
      • Engrailed/Invected antibody
      • Homeobox protein invected antibody
      • Inv antibody
      • Invected antibody
      see all

    画像

    • ab12454 used in immunohistochemistry at 1/200 on Drosophila embryos. The protocol used is available in the Neuroscience Abwire in the protocols section.

      Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining.Visualisation was with DAB.

    • ab12454 used in immunofluorescence at 1/200 on Drosophila embryos. The protocol used is available in the Neuroscience Abwire in the protocols section.

      Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining.

    参考文献

    This product has been referenced in:
    • Baratte S  et al. Engrailed in cephalopods: a key gene related to the emergence of morphological novelties. Dev Genes Evol 217:353-62 (2007). Read more (PubMed: 17394016) »
    • Harzsch S Evolution of identified arthropod neurons: the serotonergic system in relation to engrailed-expressing cells in the embryonic ventral nerve cord of the american lobster homarus americanus milne edwards, 1873 (malacostraca, pleocyemata, homarida). Dev Biol 258:44-56 (2003). Read more (PubMed: 12781681) »

    See all 11 Publications for this product

    レビューと Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample
    Fruit fly (Drosophila melanogaster) Cell (Drosophila Wing Imaginal disc)
    Specification
    Drosophila Wing Imaginal disc
    Permeabilization
    Yes - 0.5% Triton X100
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Mar 16 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録